Reimer, RayleneEslinger, Amanda2012-07-132015-07-032012-07-122012Eslinger, A. (2012). Effect of combined oligofructose and sitagliptin treatment on pre-pregnancy weight loss and gut microbiota in diet-induced obese rats (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25795http://hdl.handle.net/11023/111The purpose of this study was to evaluate a combined dietary (oligofructose) and pharmacological (sitagliptin) anti-obesity therapy in diet-induced obese female Sprague-Dawley rats and determine if the effects persist throughout pregnancy and lactation. Body weight, glucose tolerance, satiety hormones, and gut microbiota were measured following obesity induction, treatment, and lactation phases of the study. During the treatment period, rats were randomized to seven experimental groups (n=13): (1) High Fat/High Sucrose (Obese Control); (2) Lean Control (Never Obese); (3) AIN-93M (Control-Treated Obese); (4) Oligofructose (Fiber-Treated Obese); (5) Sitagliptin (Drug-Treated Obese); (6) Oligofructose and Sitagliptin (Combination-Treated Obese); and (7) Caloric Restriction (Weight-Matched to Group 6). The combination of oligofructose and sitagliptin resulted in enhanced weight loss, improved glucose tolerance, and gut hormone and microbiota profiles associated with a lean phenotype. Some, but not all, benefits persisted to the end of lactation. The combined therapy may represent a novel anti-obesity treatment.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.NutritionOligofructoseSitagliptinObesitymicrobiotaSatietymaster thesis10.11575/PRISM/25795