Coffin, CarlaCzub, MarkusLee, Zengina2014-07-172015-07-182014-07-172014Lee, Z. (2014). Hepatitis B Virus (HBV) Infection in Peripheral Blood Mononuclear Cells of HBV Mono-infected and HBV/Human Immunodeficiency Virus Type-1 Co-infected Patients (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26351http://hdl.handle.net/11023/1635It is unknown whether HIV-1 co-infection impacts HBV lymphotropism. We hypothesize that concomitant HIV-1 infection will affect HBV detection in PBMC subsets. We compared HBV genome detection in whole PBMC and CD4+ T, CD8+ T, CD14+ monocyte, CD19+ B and CD56+ NK cell subsets isolated from 14 HBV mono-infected (4/14 with a second sample collected after starting antivirals) and 6 HBV/HIV-1 co-infected patients on antivirals using nested PCR/nucleic hybridization and/or quantitative PCR assays. HBV DNA was detected in most target PBMC subsets regardless of HIV-1 co-infection and antiviral treatment; with the exception of the CD4+ T cell subset from HBV/HIV-1 + patients. All whole PBMC analyzed (13/13 HBV treatment naïve mono-infected, 4/4 follow-up cases on antivirals, and 3/3 HBV/HIV-1 co-infected) were HBV genome positive. The data suggests that HBV infection in CD4+ T cells is affected by concomitant HIV-1 infection.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.MicrobiologyHealth Care ManagementImmunologychronic HBVhuman immunodeficiency virusperipheral blood mononuclear cellPBMC subsetslymphotropismmaster thesis10.11575/PRISM/26351