Harrison, JoeDerksen, DarrenMcCartney, Nathan2017-06-132017-06-1320172017McCartney, N. (2017). Microbial natural products that inhibit c-di-GMP signalling and biofilm formation in Pseudomonas aeruginosa (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26903http://hdl.handle.net/11023/3883Pseudomonas aeruginosa is a notorious opportunistic pathogen that forms highly antibiotic resistant biofilms to establish chronic infections. Antibiotic resistance of this organism is a significant contributor to mortality in immunocompromised patients. In this thesis, a high-throughput screening protocol was used to identify microbial spent media with inhibitory activity against P. aeruginosa c-di-GMP signalling. The biocontrol agent Pseudomonas protegens Pf-5 was identified to have significant inhibitory activity against the c-di-GMP signalling and biofilm forming capacity of P. aeruginosa. The natural product secreted by P. protegens was shown to be dependent on the PSL polysaccharide produced by P. aeruginosa and may exert its effect through the dipA pathway. Results demonstrated that this natural product had activity against a range of clinically-isolated P. aeruginosa and holds promising translational benefits to the eradication of chronic biofilm infections.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.MicrobiologyBiology--MolecularPseudomonas aeruginosaNatural productsMicrobiologyanti-infectivebiofilmsAntibiotic resistancemaster thesis10.11575/PRISM/26903