Kallos, MichaelGates, IanLe, An2016-02-032016-02-032016-02-032016Le, A. (2016). Computational Fluid Dynamics Modeling of Scalable Stirred Suspension Bioreactors for Pluripotent Stem Cell Expansion (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25392http://hdl.handle.net/11023/2831Pluripotent stem cells (PSCs) including embryonic and induced pluripotent stem cells are known for their potential use in cell-based therapy, disease model study, and drug screening. One of the key challenges in pluripotent stem cell research is to establish scalable bioprocesses that reliably produce cells with high quality at any desired quantity. Stirred suspension bioreactors (SSBs) are known to provide a controlled and well-mixed environment for aggregate-forming cells, such as murine and human PSCs. Hydrodynamic environment of SSBs, particularly shear stress and small eddies, have been shown to have a significant impact on the expansion and pluripotency of pluripotent stem cells. However, the exact mechanism has not been fully understood. In this project, computational fluid dynamic (CFD) simulation was employed to model the hydrodynamic environment within SSBs with various configurations and physical conditions. Understanding the hydrodynamics is one of the first key steps in bioprocess development of PSCs using SSBs.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.Engineering--BiomedicalBioreactorCFDAggregatePluripotentScale-upScale-downEddymaster thesis10.11575/PRISM/25392