Léguillette, RenaudShields, Erin2016-12-222016-12-2220162016Shields, E. (2016). 'High-sensitivity' Cardiac Troponin-T Assay Use in Horses: Analytical and Biological Validation, Post-Race Kinetics and Sampling Guidelines (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26367http://hdl.handle.net/11023/3512’High-sensitivity’ cardiac troponin assays are now the standard in human cardiology, but validation and exercise-kinetics in horses have not been investigated. Objectives: Appropriately validate the hscTnT assay for use in horses, establish reference intervals, determine biological variation, explore race-induced hscTnT release kinetics, provide clinical sampling guidelines, and create model for sub-clinical cardiomyopathy. Methods/Results: Analytical performance of the assay in horses is verified. Reference intervals: upper 95th and 99th percentile of the hscTnT population distribution were 6.8 and 16.2ng/L in Non-Competition Horses, and 14.0 and 23.2ng/L in Racing-Thoroughbreds. Biological variation not appreciated due to number of horses below assay detection level. Racing-exercise caused peak hscTnT levels at 2-6h post-race, may approach 99th percentile URL, but declined by 12-24h. Clinician guidelines: normal horses should have declining levels by 12-24h and single values during this time-period should be <99th percentile URL:23.2ng/L. Occult sodium monensin cardiomyopathy model induced hscTnT elevations and ultrastructural cardiac changes.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.Veterinary ScienceTroponin-TCardiacHorseBiomarkermaster thesishttp://dx.doi.org/10.11575/PRISM/26367