Heyne, BelindaZaremberg, VaninaSveen, Christopher Edward2017-12-182017-12-182012Sveen, C. E. (2012). Development of a new fluorescent tool box based on tocopherol (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4924http://hdl.handle.net/1880/105925Bibliography: p. 143-153Research into Vitamin E and its in-vivo function has recently exploded, focusing mostly on the biologically relevant isomer, a-tocopherol ( a-T). Although a-T is well known for its radical scavenger role in membranes, new roles of a-T not related to its antioxidant activity have been proposed. Much of this research has been slowed down due to the lack of readily accessible tools for the study of this Vitamin in vivo. Thus, our goal was to develop a toolbox of fluorescent tocopherol derivatives, either redox-active or silent, to be used for live studies. The fluorophore of choice was nitrobenzoxadiazole (NBD). After the successful synthesis and characterization of derivatives, the efficacy of several promising probes was tested in-vivo. Structural features relevant for intracellular localization were identified. In addition, the poorly understood photochemistry of NBD was investigated using laser flash photolysis on the simple probe NBD-hexane, revealing a novel phosphorescence process not previously described.xxix, 189 leaves : ill. ; 30 cm.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.master thesis10.11575/PRISM/4924