Jurkovicova-Tarabova, BohumilaCmarko, LeosRehak, RenataZamponi, Gerald WLacinova, LubicaWeiss, Norbert2019-04-142019-04-142019-04-08Molecular Brain. 2019 Apr 08;12(1):34http://hdl.handle.net/1880/110158https://doi.org/10.11575/PRISM/45327Abstract The physiological functions controlled by T-type channels are intrinsically dependent on their gating properties, and alteration of T-type channel activity is linked to several human disorders. Therefore, it is essential to develop a clear understanding of the structural determinants responsible for the unique gating features of T-type channels. Here, we have investigated the specific role of the carboxy terminal region by creating a series a deletion constructs expressed in tsA-201 cells and analyzing them by patch clamp electrophysiology. Our data reveal that the proximal region of the carboxy terminus contains a structural determinant essential for shaping several gating aspects of Cav3.3 channels, including voltage-dependence of activation and inactivation, inactivation kinetics, and coupling between the voltage sensing and the pore opening of the channel. Altogether, our data are consistent with a model in which the carboxy terminus stabilizes the channel in a closed state.Journal Article2019-04-14enThe Author(s).https://doi.org/10.1186/s13041-019-0457-0