Sanderson, Kenneth E.Kothapalli, Sushma2005-08-162005-08-162004Kothapalli, S. (2004). Genome rearrangements in salmonella enterica serovar typhi (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/174730612975959http://hdl.handle.net/1880/41681Bibliography: p. 190-206Some pages are in colour.The order of core genes on the chromosomes of Escherichia coli K12 and of Salmonella enterica serovar Typhimurium LT2 is strongly conserved. However, the chromosome of Salmonella enterica serovar Typhi is rearranged due to homologous recombination between the rrn operons. PCR analysis detected the exact order and orientation of the seven I-Ceul fragments between operons in 136 independent wild-type strains of S. Typhi. Rearrangements within cultures of S. Typhi are common but cells with rearrangements are lost from culture. However, in spite of many rearrangements in S. Typhi, the PCR data support both the genome balance and gene dosage hypotheses for genome conservation. SARB63, a wild-type S. Typhi strain, has an unbalanced genome and a reduced growth rate in LB medium, whereas the revertents produced from SARB63 are rearranged back towards genomic balance and also have improved growth rates. Comparison of the sequenced strains of enteric bacteria for the terminus region suggests Dif as an important site in the terminus region.xxi, 206 leaves : ill. ; 30 cm.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.master thesis10.11575/PRISM/17473AC1 .T484 2004 K68