Lewis, Ian A.Nguyen, Austin2019-09-232019-09-232019-09-19Nguyen, A. (2019). Critically Re-evaluating Carbohydrate Metabolism in Pseudomonas aeruginosa (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.http://hdl.handle.net/1880/111034Several studies have linked carbon metabolism to a variety of different factors in P. aeruginosa. For example, the production of gluconate and 2-ketogluconate has been linked to exotoxin production, antibiotic resistance, and iron acquisition. However, glucose catabolism is known to participate in P. aeruginosa aerobic respiration. We hypothesize that, rather than factors such as iron acquisition, P. aeruginosa gluconate and 2-ketogluconate production is instead driven by the energetic needs of the cell. We developed a targeted LC-MS method and analyzed P. aeruginosa glucose metabolism production in a variety of limiting conditions. From our data, we made a model of P. aeruginosa metabolism where energy production is decoupled from biomass generation via periplasmic glucose oxidation. This model introduces a regulatory mechanism of carbon catabolism that explains the production of gluconate and 2-ketogluconate through cellular energy demands.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.MetabolismMetabolomicsPseudomonas aeruginosaGlucoseLC-MSEntner-Doudoroff PathwayGluconate2-ketogluconateMicrobiologyBiochemistrymaster thesis10.11575/PRISM/37096