Chaconas, GeorgeBandy, Nick2013-10-092013-11-122013-10-092013Bandy, N. (2013). Construction and Characterization of a B. burgdorferi strain with Conditional Expression of the Essential Telomere Resolvase, ResT (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25430http://hdl.handle.net/11023/1139During DNA replication in Borrelia burgdorferi, replication of linear elements results in an inverted dimer repeat of the telomere which is subsequently resolved by the telomere resolvase, ResT. ResT has been demonstrated as essential and a knock-out strain has never been produced. Using a conditional expression system a resT knock-out strain has been produced and used to investigate the effects of ResT depletion of spirochete viability as well as genome stability. ResT depleted spirochetes ceased to divide after ResT depletion, however, remained viable up to 16 days suggesting an arrested state of the spirochetes. Southern blots of the DNA revealed a shift of linear plasmid DNA to a higher molecular weight representing the circular intermediate. DNA replication did not continue in spirochetes after ResT depletion suggesting ResT may play a larger role in replication than previously thought.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.GeneticsMicrobiologyBiochemistryBorreliaTelomereLymemaster thesis10.11575/PRISM/25430