Cramb, David ThomasBishop, Amanda Iris2018-07-122018-07-122018-07-10Bishop, A. I. (2018). Interactions between Serum Albumin Proteins and Polystyrene Nanoparticles (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/32363http://hdl.handle.net/1880/107141Nanoparticles (NPs) have become increasingly popular for several applications, especially regarding biomedical applications because of their unique properties. However, when a nanoparticle enters a biological medium, it is thought to become encapsulated in proteins and other biomolecules in a coating termed a “protein corona.” This coating is significant as it can change the identity and surface properties of the nanoparticle, thus affecting its fate within the biological medium. Studies on the formation of NP-protein complexes have been ongoing for years although the interactions are still not fully understood due to their dynamics and complexity. As a result, this hinders the use of NPs to their full potential in biomedical applications. The studies performed in this thesis analyze the interactions of fluorescent polystyrene nanoparticles (FS) of two different sizes with bovine serum albumin proteins (BSA) by the technique of Two-Photon Excitation Cross-correlation Spectroscopy (TPE-FCCS). These interactions were explored both thermodynamically and kinetically to gain insight into the formation of the early hard corona and the kinetics of the formation of the BSA-FS complexes. The results suggested very low binding ratios and a mechanism of protein association dependent on the size of the sphere present in solution. The results also suggested an irreversible formation of BSA-FS complexes, in which the BSA appears to stack at the surface of the FS. These findings are significant as they challenge the current beliefs on the formation of a protein corona and perceived monolayer formation, and furthermore, provides a deeper understanding of NP-protein interactions.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.Nanoparticlesserum albumin proteinspolystyrene nanoparticlesprotein coronatwo-photon excitationEducation--Sciencesmaster thesis10.11575/PRISM/32363