van Marle, GuidoMintchev, Paul2015-07-092015-11-202015-07-092015Mintchev, P. (2015). Loop-Mediated Isothermal Amplification for Diagnosis of Major Infectious Diseases in Resource-Limited Settings (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26788http://hdl.handle.net/11023/2341Major Infectious Diseases (MIDs) continue to pose major global health-care challenges in 2015, particularly in resource-limited settings. Alongside the recent global Human Immu-nodeficieny Virus (HIV)/Acquired Immunodeficieny Syndrome (AIDS) pandemic, there has been a marked resurgence in fatalities related to Malaria and Leishmaniasis in Lower-to-Middle-Income Countries (LMICs). With the recent development of Loop-Mediated Isothermal Amplification (LAMP), researchers are hoping to bring rapid and inexpensive point-of-care diagnostics, based on nucleic acid amplification, to LMIC settings. We ex-plored the possibility of a portable, low-cost LAMP-based diagnostic device targeting HIV, Malaria, and Leishmaniasis, for use in resource-limited settings, in an idealized proof-of-concept study. Design and build an inexpensive functional prototype with the potential to quantify disease severity that demonstrates the feasibility of this diagnostic tool for the desired application was the primary outcome; however, much work remains to be done in order to optimize the method to a reliable and repeatable clinical tool.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.Engineering--BiomedicalBiomedical EngineeringMedical DiagnosticsMicrobiologymaster thesis10.11575/PRISM/26788