Cramb, DavidBlades, Megan2015-02-062015-02-062015http://hdl.handle.net/11023/2090Expanding on theoretical work by Heinze et al.1, an optical setup to experimentally realize three-colour fluorescence cross-correlation spectroscopy (3C-FCCS) was designed. This instrument simultaneously collects and correlates fluorescence data from three colour channels, allowing real-time tracking of three-coloured species in complex solutions. To examine 3C-FCCS theory, a nanobarcode test particle, which physically linked three fluorophores was selected and characterized. These nanobarcode particles, capable of producing a triple cross-correlation decay, facilitated proof-of-concept experiments. 3C-FCCS was shown to be capable of measuring the concentration of nanobarcode particles in solution exclusively containing nanobarcode particles and solution containing nanobarcodes outnumbered by background quantum dots at a ratio of 800:1. 3C-FCCS was used to investigate a simple DNA-based nanostructure. These experiments illustrate that 3C-FCCS is a compelling technique with a plethora of potential applications, ranging from investigating hierarchical nanoassembly to biological reactions.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.Chemistry--PhysicalFluorescence Cross-Correlation Spectroscopymaster thesis10.11575/PRISM/28052