Rohatensky, Mitchell GLivingstone, Devon MMintchev, PaulBarnes, Heather KNakoneshny, Steven CDemetrick, Douglas JDort, Joseph Cvan Marle, Guido2018-02-132018-02-132018-02-08Rohatensky, M. G., Livingstone, D. M., Mintchev, P., Barnes, H. K., Nakoneshny, S. C., Demetrick, D. J., ... & van Marle, G. (2018). Assessing the performance of a Loop Mediated Isothermal Amplification (LAMP) assay for the detection and subtyping of high-risk suptypes of Human Papilloma Virus (HPV) for Oropharyngeal Squamous Cell Carcinoma (OPSCC) without DNA purification. BMC Cancer, 18(1), 166.1471-2407http://hdl.handle.net/1880/106394https://doi.org/10.11575/PRISM/43847Calgary Surgical Research Development Fund, the Ohlson Research Initiative, Alberta Innovates Health SolutionsOropharyngeal Squamous Cell Carcinoma (OPSCC) is increasing in incidence despite a decline in traditional risk factors. Human Papilloma Virus (HPV), specifically subtypes 16, 18, 31 and 35, has been implicated as the high-risk etiologic agent. HPV positive cancers have a significantly better prognosis than HPV negative cancers of comparable stage, and may benefit from different treatment regimens. Currently, HPV related carcinogenesis is established indirectly through Immunohistochemistry (IHC) staining for p16, a tumour suppressor gene, or polymerase chain reaction (PCR) that directly tests for HPV DNA in biopsied tissue. Loop mediated isothermal amplification (LAMP) is more accurate than IHC, more rapid than PCR and is significantly less costly. In previous work we showed that a subtype specific HPV LAMP assay performed similar to PCR on purified DNA. In this study we examined the performance of this LAMP assay without DNA purification.enLAMPHPVOPSCCLoop-mediated isothermal amplificationOropharynxjournal articlehttp://dx.doi.org/10.1186/s12885-018-4087-1