Wieser, Michael E.Karchewski, BrandonWilkins, Aaron Francis2020-05-152020-05-152020-05-12Wilkins, A. F. (2020). Inversion Modelling of Copper Transport in Saccharomyces Cerevisiae (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.http://hdl.handle.net/1880/112058Copper is an essential nutrient but the uptake into cells is poorly understood. This dissertation summarizes the development of a mathematical system of equations to model the transport of copper in S. cerevisiae. Yeast is a model organism for studying the copper transport in human hepatic cells because the chaperone proteins and structures are well conserved between the species. An experiment is performed to investigate the transport between the growth media and the cells to model the process behind this important pathway. Transport mechanisms for this process are presented, mathematically modelled, and evaluated. Rate limited diffusion did not appear to be adequate in modelling the transport, but a term including a target copper concentration which cells actively maintain was introduced, and with a delayed activation, fit the data much more effectively. With this model, a framework is established for incorporating organelles to eventually model the intracellular copper transport and analyze the copper isotope distributions in the future. This work contributes to a larger initiative to incorporate copper isotope analysis as an innovative medical diagnostic tool in assessing human cellular pathology.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.Analytical chemistryInversion problemsInverseSaccharomyces CerevisiaeCopperStable IsotopesLogisticIsotope Dilution Mass SpectrometryMass SpectrometryMC-ICP-MSModellingCopper transportYeastIDMSEducation--MathematicsEducation--SciencesBiophysicsGeochemistryChemistry--AnalyticalBiochemistryPhysicsPhysics--MolecularApplied Sciencesmaster thesis10.11575/PRISM/37845