Mains, Paul E.Johnson, Jacque-Lynne F.A.2005-08-162005-08-162004Johnson, J. L. (2004). Characterization of the mel-26, the post-meiotic inhibitor of MEI-1/MEI-2 microtubule severing activity in caenorhabditis elegans (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/121400612977137http://hdl.handle.net/1880/41638Bibliography: p. 109-127Some pages are in colour.In the Caenorhabditis elegans embryo, the meiotic and mitotic spindles form within thirty minutes of one another in the same cytoplasm; yet these two spindles are very different in size and structure. Genes have been identified that have an essential role in oocyte meiotic and early mitotic spindle formation. It was previously determined that mei-1 and mei-2 are essential for meiosis and that mel-26 is the post-meiotic inhibitor of mei-1 and mei-2. mei-1 and mei-2 encode a microtubule severing activity. This thesis focuses on the development of an anti-MEL-26 antibody, to determine where and when MEL-26 is localized in the early embryo and to discover more about its interaction with MEI-1/MEI-2. This work contributed to the identification of MEL-26 as a component of a novel E3 ubiquitin ligase complex involved in targeting MEI-1 for ubiquitin-mediated degradation following meiosis.xii, 127 leaves : ill. ; 30 cm.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.master thesishttp://dx.doi.org/10.11575/PRISM/12140AC1 .T484 2004 J645