Browsing by Author "ALGHAMDI, ENAAM"

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    Force Based Comparison Between Naïve and Activated CD4+ T Cells during Interaction with Dendritic Cells
    (2014-04-11) ALGHAMDI, ENAAM; Shi, Yan
    Antigen recognition requires the communication between T cells and antigen presenting cells (APC) such as dendritic cells (DC). Although there is progress in understanding molecular components at the intercellular contact, the specific role of the physical cross-junctional adhesion between CD4+ T cells and DCs is not fully understood. In this study, we investigate cell-to-cell contact mediated by adhesion molecules between these cells in order to elicit the mechanism involved. Lymphocyte function-associated antigen-1 (LFA-1) adhesion molecule on the surface of T cells interacts with intracellular adhesion molecule-1 (ICAM-1) on the APC. This adhesion pair has been shown to be essential for the contact between the cells. The role of this adhesion pair and its expression and regulation on naïve CD4+ T cells has not been compared to its role on activated T cells. Using atomic force microscopy (AFM) we investigated the increase in interaction forces between naïve CD4+ T cells and DCs. These forces were compared to those involving activated T cells to see the difference between naïve and activated T cells in their adhesion properties with DCs, which involves LFA-1 integrin molecule. Understanding the role of LFA-1 interaction with ICAM-1 provides further insight into therapies using CD4+ T cells.