Browsing by Author "Barrette-Ng, Isabelle H."

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    The Mentorship Guide for Teaching and Learning
    (Taylor Institute for Teaching and Learning, 2019) Barrette-Ng, Isabelle H.; Nowell, Lorelli; Anderson, Sarah J.; Arcellana-Panlilio, Mayi; Brown, Barbara; Chalhoub, Serge; Clancy, Tracey L.; Desjardine, Patricia; Dorland, AnneMarie; Dyjur, Patti; Mueller, Katherine; Reid, Leslie; Squance, Rod T.; Towers, Jo; Wilcox, Gabrielle
    Rooted in evidence, this guide will provide you with a unique perspective on supporting mentoring relationships for teaching and learning development. You will explore mentoring relationships, assessing readiness for mentorship, initiating mentorship, developing and sustaining mentoring relationships and mentoring transitions while ultimately improving student learning. You will also reflect on your mentoring relationships as you work through a series of guided questions and practical worksheets. Research on academic mentorship often measures success in terms of mentee research productivity (Feldman, Arean, Marshall, Lovett & O’Sullivan, 2010; Kalet, Fletcher, Ferdman & Bicknell, 2006; Sambunjak, Straus & Marušić, 2006). Teaching mentorship produces different measures of success, including the development of reflective practice and, most importantly, improvements in student learning. Although there are many resources for mentorship in academia (Johnson, 2015; Straus & Sackett, 2014), none of them aim specifically at supporting mentorship for teaching and learning development. Mentorship in teaching and learning differs from research mentorship in terms of aims, approaches and measures of success, which is why we offer this resource.
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    Utilization of plant Puf RNA-binding proteins to manipulate endogenous mRNA physiology
    (2019-02-01) Wen, Xin; Muench, Douglas G.; Hansen, David V.; Barrette-Ng, Isabelle H.
    RNA-binding proteins have an important role in the regulation of post-transcriptional gene expression. The Puf (Pumilio) family of RNA-binding proteins bind to RNAs in a sequence specific manner. The RNA-binding domain of canonical Puf proteins (the Pumilio homology domain, PUM-HD) consists of eight Puf repeats. Each Puf repeat binds to RNA in a modular fashion, so that one Puf repeat binds to a single nucleotide. The nucleotide binding recognition code of Puf repeats has been well characterized. This one repeat:one nucleotide recognition code has allowed for the alteration of Puf repeats so that the PUM-HD can bind specific RNA targets. These altered Puf repeats have been fused to effector domains so that fusion proteins can affect RNA physiology in the cell. The aim of this thesis was to fuse an Arabidopsis Puf RNA-binding protein with unique binding characteristics to two types of effector domains. These fusion proteins were expressed in onion epidermal cells to determine if they could alter the stability or translation of a reporter mRNA. This research provides foundational evidence that highlights the potential for the utility of plant Puf proteins to alter gene expression for applied purposes