Browsing by Author "Behie, Leo A."
Now showing 1 - 20 of 45
Results Per Page
Sort Options
Item Open Access A chemical reaction kinetic study into the low severity pyrolysis of Athabasca bitumen(1998) Wilcott, Heather R.; Behie, Leo A.Item Open Access A predictive model for a circulating fluidized bed riser reactor(1998) Puchyr, David Michael John; Behie, Leo A.Item Open Access An experimental and modeling study of homogeneous gas phase reactions occurring in the modified claus process(1998) Karan, Kunal; Behie, Leo A.Item Open Access Bioprocessing of human breast and brain cancer tissue(2009) Panchalingam, Krishna M.; Behie, Leo A.Item Open Access Bioprocessing of human embryonic stem cells for the treatment of Parkinson's disease(2008) Williams, Laura A.; Behie, Leo A.Item Open Access Bioreactor expansion of human neural precursor cells for the treatment of neurodegenerative disorders(2008) Ahmadian Baghbaderani, Behnam; Behie, Leo A.Item Open Access Bioreactor protocols for the long-term expansion of mammalian neural stem cells in suspension culture(2003) Sen, Arindom; Behie, Leo A.Item Open Access Cancer stem cell bioreactor scale-up(2008) Youn, Benjamin; Behie, Leo A.Item Open Access Cell cycle production of monoclonal antibody from hybridoma and recombinant myeloma cell lines(1993) Farrell, Patrick James; Behie, Leo A.Item Open Access Computer simulation of an industrial quench cooler on a large ethane cracking furnace(1988) Huntrods, Richard S.; Behie, Leo A.Item Open Access Developing large-scale bioreactor protocols to expand pancreatic endocrine tissue to treat type 1 diabetes(2004) Chawla, Meera; Behie, Leo A.Type 1 diabetes is a disease of the pancreas in which the beta islet (endocrine) cells, which are responsible for insulin production, are destroyed by the immune system. The expansion in bioreactors and subsequent transplantation of endocrine cells could revolutionize the treatment for Type 1 diabetes using the "world-famous" Edmonton Protocol. Consequently, the aim of this study was to develop a scaleable process to expand mammalian pancreatic endocrine tissue in suspension bioreactors. Issues regarding cell culture medium conditions and cell handling protocols were investigated. Experiments in suspension bioreactors for 9 days showed a greater than 7-fold increase in the number of insulin-positive cells. Furthermore, aggregates were islet-like, as all of the endocrine cell types were present. Most importantly, cells exhibited glucose-responsive behavior and thus functionality.. These results represent a major milestone on the path to effective expansion and clinical use of bioreactor-produced islet-like structures in the treatment of Type 1 diabetes.Item Open Access Dynamic modelling of a sprouted bed reactor with a draft tube used for hydrocarbon ultrapyrolysis(1988) Eng, John Harvey; Behie, Leo A.Item Open Access Dynamic modelling of an immobilized enzyme bioreactor(1987) Papathanasiou, Athanasios; Behie, Leo A.Dynamic experiments provide an excellent means for the determination of crucial process parameters, such as the axial Peclet number, the intraparticle and external mass transfer coefficient and the intraparticle reaction rate constant, in fixed or liquid fluidized bed immobilized enzyme bioreactors. The absence of a complete and comprehensive solution of the model equations in the real time domain, as well as significant advances in the area of statistical moments analysis has rendered the latter approach the dominant one for parameter estimation in fluid-solid reacting systems. Nevertheless, an efficient and easily implementable solution of the model equations in the real time domain, as opposed to the solution in the Laplace domain needed for the statistical moments analysis, opens new possibilities in the design of fixed or fluidized bed bioreactors since, apart from parameter estimation via dynamic experiments, it is suitable for simulation, optimization and control. This paper presents a dynamic model for a fixed or liquid fluid bed immobilized enzyme bioreactor, along with a novel method for the solution of the coupled partial differential equations in the real time domain. Both, the tanks-inseries and the dispersion models have been used to describe the non ideal axial mixing in the reactor. The solution, in its final form, comes in both cases as a system of simultaneous ordinary differential equations; this is readily implementable on a computer and can be easily solved by commercially available software packages. Based on this solution, a complete parametric analysis was performed. That analysis revealed the importance of intraparticle and external mass transfer resistances, intraparticle chemical reactio?? and axial dispersion on the transient behaviour of the reactor. Most important, that analysis revealed ways for parameter estimation and system identification via simple dynamic experiments. The design and optimization implications of the study are finally demonstrated by using the derived solution to simulate the performance of an immobilized urease bioreactor with a recycle loop. Such a configuration is characterized by time varying feed concentration and can be used, as part of an extracorporeal artificial kidney device, for the treatment of uremic patients.Item Open Access Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media(Hindawi Publishing Corporation, 2012-01-31) Jung, Sunghoon; Panchalingam, Krishna M.; Rosenberg, Lawrence; Behie, Leo A.Item Open Access Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media(2012-05-07) Jung, Sunghoon; Panchalingam, Krishna M.; Rosenberg, Lawrence; Behie, Leo A.Human mesenchymal stem cells (hMSCs) are presently being evaluated for their therapeutic potential in clinical studies to treat various diseases, disorders, and injuries. To date, early-phase studies have indicated that the use of both autologous and allogeneic hMSCs appear to be safe; however, efficacy has not been demonstrated in recent late-stage clinical trials. Optimized cell bioprocessing protocols may enhance the efficacy as well as safety of hMSC therapeutics. Classical media used for generating hMSCs are typically supplemented with ill-defined supplements such as fetal bovine serum (FBS) or human-sourced alternatives. Ideally, culture media are desired to have well-defined serum-free formulations that support the efficient production of hMSCs while maintaining their therapeutic and differentiation capacity. Towards this objective, we review here current cell culture media for hMSCs and discuss medium development strategies.Item Open Access Expansion and characterization of human pancreas derived precursor cells(2006) Bodnar, Cheryl; Behie, Leo A.Item Open Access Expansion of human brain cancer derived tumour tissue for the development of the new therapeutic strategies(2008) Chiang, Chun-Yi Katherine; Behie, Leo A.Item Open Access Growth characteristics of a bombyx mori insect cell line in stationary & suspension cultures(1990) Stavroulakis, Dimitrios Angelos; Behie, Leo A.Item Embargo Growth characteristics of a bombyx mori insect cell line in stationary and suspension cultures(1990) Stavroulakis, Dimitrios Angelos; Behie, Leo A.Item Open Access High efficiency bioreactor technology for recombinant protein production using a baculovirus/insect cell (BmNPV/Bm5) system(1993) Zhang, Junli; Behie, Leo A.
- «
- 1 (current)
- 2
- 3
- »