Browsing by Author "David, Joel"
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- ItemOpen AccessGene Expression Study Of Mycobacterium avium subspecies paratuberculosis Infected Cows – “A road to identify transcripts that could serve as biomarkers for early diagnosis of Johne’s disease”(2014-02-05) David, Joel; De Buck, Jeroen; Barkema, Herman; Ghosh, Subrata; Guan, LeLuoCurrent diagnostic tools lack sensitivity to detect JD early after infection. Hence, this study aimed to find biomarkers for early diagnosis of JD. An infection trial was set up with a high dose (n=5) and low dose (n=5) oral challenge with Mycobacterium avium subsp. paratuberculosis at 2 weeks of age and 5 non-infected control animals. Whole blood was collected at a 3-month interval (3, 6, 9, 12 and 15 months) for gene expression analysis using Affymetrix® GeneChip® Bovine Genome Array on samples obtained at 3, 6 and 9 months after infection. Microarray findings were confirmed using qPCR and longitudinal assays profiling transcripts over 15-month period was carried out. This study found differential gene expression at each sampled time point and found transcripts for potential biomarker use. CD46 was upregulated and BOLA and BNBD9-like genes were downregulated over the entire 15-months. Overall immune response was inhibited in MAP-challenged animals.