Browsing by Author "Maslieieva, Valentyna"

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    Activation of Epiplexus Cells by ATP
    (2013-01-25) Maslieieva, Valentyna; Thompson, Roger John
    Epiplexus cells represent a population of innate immune cells on the surface of the choroid plexus (CP) in the brain ventricles. I hypothesized that the epiplexus cells are involved in immune responses of the CP via sensitivity to immune mediators such as ATP. A novel technique for the isolation of live and intact rat CP was developed that allowed, for the first time, observation and quantification of physiological responses of epiplexus cells in situ. Bath application of ATP, as well as ADP or UTP, activated movement (chemokinesis) of epiplexus cells. This was not dependent upon P2X7 and P2X4 receptors, but may involve epiplexus cell P2X1, P2X5, and / or P2Y13 and P2Y2 receptors and epithelial cell pannexin-1. Therefore, epiplexus cells respond to the immune mediator, ATP, and may receive signals directly from the epithelium on which they reside to mediate localized immune responses in the ventricle.