Browsing by Author "McGhee, James D."
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Item Open Access A neural specification gene in C. elegans(1994) Svendsen, Pia C.; McGhee, James D.Item Open Access Carboxylesterases from the nematode Panagrellus redivivus(1998) Culp, Shelley A.; McGhee, James D.Item Open Access Characterization of the C. elegans erlin homologue(BioMed Central, 2012-01-23) Hoegg, Maja B.; Robbins, Stephen M.; McGhee, James D.Item Open Access Early development in nematodes(1994) Azzaria, Marie; McGhee, James D.Item Open Access gob-1 is a trehalose-6-phosphate phosphatase required for intestine development in the nematode caenorhabditis elegans(2005) Kormish, Jay Dene; McGhee, James D.Item Open Access gob-1 is a trehalose-6-phosphate phosphatase required for intestine development in the nematode caenorhabditis elegans(2004) Kormish, Jay Dene; McGhee, James D.Item Open Access gob-1 is a trehalose-6-phosphate phosphatase required for intestine development in the nematode caenorhabditis elegans(2004) Kormish, Jay Dene; McGhee, James D.A genetic screen was developed with the intention of finding C. elegans genes that are required for intestine development or differentiation. The screen was based on the gut obstructed phenotype of the elt-2 null mutant. elt-2 is a GATA transcription factor believed to be the major transcription factor driving intestine differentiation. It was anticipated that screening for a similar gut obstructed mutant phenotype could identify genes that were functioning redundantly to elt-2 or downstream of elt-2. One of the mutations identified in this screen, gob-1(ca17), was mapped to the right-hand side of the X chromosome. Positional cloning of the gob-1(ca17) allele showed that it was a deletion of approximately 15 open reading frames. RNA-mediated interference to a gene in this region, H13N06.3, phenocopied the early larval arrest and gut obstructed phenotype of gob-1(ca17) and was thus identified as the gene responsible for this phenotype. The gob-1 RNAi mutant causes an early defect in the intestine. The gob-1 gene and GOB-1 protein are expressed specifically in the intestine during its early development. GOB-1 is a member of the Haloacid dehalogenase (HAD)-like hydrolase superfamily and is the first identified trehalose-6-phosphate phosphatase in the nematode phylum. GOB-1’s function in trehalose metabolism is currently unclear but mounting evidence suggests that GOB-1 may have a function required for viability of the worm independent of trehalose synthesis. ELT-2 is sufficient but not necessary for early embryonic expression of GOB-1. The ability of ELT-2 to drive gob-1 expression suggests that loss of gob-1 iv function may be a component of the later elt-2 loss of function defect in the larval intestine.Item Open Access Homeobox genes in Caenorhabditis elegans(1989) Hawkins, Nancy C.; McGhee, James D.Item Open Access Isolation and characterization of a gut-specific acid phosphatase in the nematode caenorhabditis elegans(1989) Beh, Christopher T.; McGhee, James D.Item Open Access Parental DNA segregation during early embryonic development(1990) Ito, Kenichi; McGhee, James D.Item Open Access Regulation of ges-1 expression in the Caenorhabditis elegans digestive tract(1998) Schroeder, Dana Frances; McGhee, James D.Item Open Access Regulation of the ges-l gene from the nematode Caenorhabditis briggsae(1998) Marshall, Sean David Goldie; McGhee, James D.Item Open Access Spatial regulation of pho-1 expression in the caenorhabditis elegans intestine(2007) Yan, Jie; McGhee, James D.Item Open Access Stimulation of a metabolic bypass for the degradation of Gm2 ganglioside in Tay-Sachs disease(2005) Kadir, Hakan; Gravel, Roy; McGhee, James D.Item Open Access Tissue-Specific Regulation of Contraction and Elongation in the C. elegans Early Embryo(2018-08-20) Drewnik, Elizabeth Daisy; Mains, Paul E.; McGhee, James D.; Brook, William J.; Cobb, John A.Morphogenesis, a key feature of embryonic development, is driven by actin cytoskeleton contraction. Actin and myosin activity promote elongation of the Caenorhabditis elegans embryo from a ball of cells to a tubular worm. I used mutants in the elongation/contraction pathway to assess tissue-specific requirements of components regulating the contractile pathway. Contraction occurs mainly in the lateral embryonic epidermal cells, while the dorsoventral epidermal cells play a more passive role. Elongation is regulated by Rho kinase/LET-502, p-21 activating kinase/PAK-1, and myosin phosphatase/MEL-11. Though it is known that LET-502 and MEL-11 are expressed throughout the epidermis, in which cells the genes are necessary or sufficient is unknown. I used transgenic animals and body length measurements to show that LET-502 is sufficient in lateral but not dorsoventral cells, and that PAK-1 and MEL-11 are not sufficient in either lateral or dorsovetnral cells.