Browsing by Author "Narsingani, Karim"

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    Investigation of Depolarization as the Trigger for Lubricin and Hyaluronan Secretion from Non-Excitable Human Synovial Fibroblasts
    (2017) Narsingani, Karim; Giles, Wayne; Schmidt, Tannin; Benseler, Susanne; Braun, Andrew
    Hyaluronan (HA) and Lubricin are major components of synovial fluid that are produced by synovial fibroblasts. Their secretion pathway remains unclear. However, calcium has been shown to play a major role. Depolarization may increase intracellular calcium. This study hypothesized that depolarization may lead to an increase in HA and Lubricin. Human synovial fibroblasts (HSF) from two donors were depolarized with 100mM extracellular K+. The media was analyzed for HA and Lubricin using and HA ELISA and AlphaLISA, respectively. Calcium fluorescence determined intracellular calcium concentration ([Ca2+]i). A generalized estimating equation (GEE) was used for statistical analysis. In the high K+ group HA concentration decreased (p=0.000); however, the Lubricin concentration did not change (p=1.000) and [Ca2+]i decreased. Depolarization with 100mM K+ for 12h did not increase [Ca2+]i, Lubricin levels and decreased HA levels in the HSF conditioned media. Results may indicate K+ is not the ion responsible for the resting membrane potential.