Browsing by Author "Naugler, Christopher T."
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Item Open Access Association of vitamin D status with socio-demographic factors in Calgary, Alberta: an ecological study using Census Canada data(BioMed Central, 2013-04-08) Naugler, Christopher T.; Zhang, Jianguo; Henne, Dan; Woods, Paul; Hemmelgarn, Brenda RItem Open Access Current usage and future trends in gross digital photography in Canada(BioMed Central, 2014-01-14) Horn, Christopher L; DeKoning, Lawrence; Klonowski, Paul; Naugler, Christopher T.Item Open Access Incidence and Risk Factors for Hyponatremia in Patients Newly Prescribed Citalopram(2019-04-26) Shysh, Andrea Christine; Naugler, Christopher T.; Sidhu, Davinder; Ismail, ZahinoorHyponatremia is a common and under-recognized adverse drug reaction of citalopram. This study aims to determine the incidence of hyponatremia and to identify risk factors in a large, population-based cohort initiating new prescriptions for citalopram. Following approval from the ethics review board, data were obtained from Alberta Information Network databases to identify patients with new citalopram prescriptions from 2010-2017, inclusive. Hyponatremia was defined as serum sodium level <135 mmol/L. Associations were determined by performing Cox regression with time-varying covariate analysis, with the development of hyponatremia as the dependent variable. This is the first large-scale, population-based study to explore risk factors, based solely on laboratory serum data, for the development of hyponatremia in patients initiating citalopram therapy. We report a 16.7% incidence of hyponatremia after starting citalopram treatment and significant risk factors include lower baseline sodium, concurrent thiazide diuretic use, older age, and male sex.Item Open Access Measuring steroids from dried blood spots using tandem mass spectrometry to diagnose congenital adrenal hyperplasia(2018-09-10) Qasrawi, Deema Omar; Sadrzadeh, S. M. Hossein; Khan, Faisal Masood; Naugler, Christopher T.; Lewis, Ian A.Congenital adrenal hyperplasia (CAH) is a group of autosomal recessive disorders that occur due to defects in the steroidogenesis pathway within the adrenal glands. CAH is characterized by numerous clinical manifestations resulting from dysregulation of various enzymes in the steroidogenesis pathway. Approximately 90% of CAH cases can be diagnosed by the measurement of serum 17-hydroxyprogesterone alone. However, the quantification of six additional steroids, cortisol, 21-deoxycortisol, 11-deoxycortisol, androstenedione, pregnenolone and dehydroepiandrosterone could significantly improve CAH laboratory diagnosis. Although clinical laboratories predominantly use immunoassays to measure some of the above steroids, these assays are hampered by low specificity due to cross reactivity of antibodies to molecules with similar structures and high cost of antibodies used in the system. As CAH is diagnosed in neonates, using dried blood spot (DBS) as specimen of choice can further improve patient care due to the very small sample volume. This study aimed to develop a more specific and rapid assay to measure seven steroids using liquid chromatography mass spectrometry (LC-MS/MS) in DBS. An optimized DBS sample preparation method was employed without the need of derivatization. A LC-MS/MS assay was developed and optimized using reverse phase-ultra performance liquid chromatography (RP-UPLC) system combined with a triple quadrupole mass spectrometry using positive electrospray ionization (+ESI) mode. Each steroid was quantified using its deuterated or isotopically labelled internal standard. Calibration curve and quality control specimens were prepared in saline-washed erythrocytes mixed with steroid free serum to achieve a 55±1% hematocrit level. Prepared specimens were enriched with predetermined concentrations of the seven steroids and spotted onto Whatman 903® filter papers. The assay was validated according to CLSI analytical guidelines, including limit of detection (LOD) and lower limit of quantification (LLOQ), linearity, precision, and method comparison. The analytical measuring range of the method for all steroids was 2.5 were 0.11-1.8 ng/ml, 0.001-6.4 ng/ml, 1.8–11.5%, and 5.3-13.8%, respectively. This robust and inexpensive assay can be readily implemented in clinical laboratories equipped with LC-MS/MS and can provides superior analytical performance over traditional immunoassays for the diagnosis of CAH.Item Open Access Morphometric characteristics of basal cell carcinoma peritumoral stroma varies among basal cell carcinoma subtypes(BioMed Central, 2012-03-09) Naugler, Christopher T.; Lesack, KyleItem Open Access Performance of a simple chromatin-rich segmentation algorithm in quantifying basal cell carcinoma from histology images(BioMed Central, 2012-01-17) Lesack, Kyle; Naugler, Christopher T.Item Open Access Population genetics of cancer cell clones: possible implications of cancer stem cells(BioMed Central, 2010-11-09) Naugler, Christopher T.Item Open Access Unsatisfactory rates vary between cervical cytology samples prepared using ThinPrep and SurePath platforms: a review and meta-analysis(BMJ, 2012-04-13) Fontaine, Daniel; Narine, Nadira; Naugler, Christopher T.