Browsing by Author "Wang, Li"

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    Developmental changes in potassium currents in mouse heart
    (1996) Wang, Li; Duff, Henry J.
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    E. coli diversity: low in colorectal cancer
    (2020-04-06) Tang, Le; Zhou, Yu-Jie; Zhu, Songling; Liang, Gong-Da; Zhuang, He; Zhao, Man-Fei; Chang, Xiao-Yun; Li, Hai-Ning; Liu, Zheng; Guo, Zhi-Rong; Liu, Wei-Qiao; He, Xiaoyan; Wang, Chun-Xiao; Zhao, Dan-Dan; Li, Jia-Jing; Mu, Xiao-Qin; Yao, Bing-Qing; Li, Xia; Li, Yong-Guo; Duo, Li-Bo; Wang, Li; Johnston, Randal N; Zhou, Jin; Zhao, Jing-Bo; Liu, Gui-Rong; Liu, Shu-Lin
    Abstract Background Escherichia coli are mostly commensals but also contain pathogenic lineages. It is largely unclear whether the commensal E. coli as the potential origins of pathogenic lineages may consist of monophyletic or polyphyletic populations, elucidation of which is expected to lead to novel insights into the associations of E. coli diversity with human health and diseases. Methods Using genomic sequencing and pulsed field gel electrophoresis (PFGE) techniques, we analyzed E. coli from the intestinal microbiota of three groups of healthy individuals, including preschool children, university students, and seniors of a longevity village, as well as colorectal cancer (CRC) patients, to probe the commensal E. coli populations for their diversity. Results We delineated the 2280 fresh E. coli isolates from 185 subjects into distinct genome types (genotypes) by PFGE. The genomic diversity of the sampled E. coli populations was so high that a given subject may have multiple genotypes of E. coli, with the general diversity within a host going up from preschool children through university students to seniors. Compared to the healthy subjects, the CRC patients had the lowest diversity level among their E. coli isolates. Notably, E. coli isolates from CRC patients could suppress the growth of E. coli bacteria isolated from healthy controls under nutrient-limited culture conditions. Conclusions The coexistence of multiple E. coli lineages in a host may help create and maintain a microbial environment that is beneficial to the host. As such, the low diversity of E. coli bacteria may be associated with unhealthy microenvironment in the intestine and hence facilitate the pathogenesis of diseases such as CRC.
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