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Pseudomonas aeruginosa Exopolysaccharide Biosynthesis and Immunosuppressive Role in Vivo

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Advisor
Harrison, Joe
Author
Rich, Jacquelyn
Committee Member
Morck, Doug
Yipp, Bryan
Other
Pseudmonas
Biofilm
Exopolysaccharide
Inflammation
Neutrophil
PEL
Epimerase
Subject
Education--Sciences
Genetics
Microbiology
Biology--Molecular
Immunology
Type
Thesis
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Abstract
Pseudomonas aeruginosa is a pathogen that can colonize the lungs of cystic fibrosis patients leading to chronic infections that are thought to have biofilm etiology. This opportunistic bacterium produces three exopolysaccharides found in the biofilm matrix: PEL, PSL and alginate. In this thesis, understanding the synthesis and expression of PEL was conducted by developing complementation vectors for each gene in the pel operon, as well as proposed genes involved in precursor synthesis. These vectors can be manipulated to learn more about how the proteins function to contribute to PEL expression, as shown using the PelA complementation vector to dissect individual residue functions. Results also suggest that the epimerases, GalE and PA4068, contribute to the synthesis of PEL. It was also found that in vivo, PEL and PSL act as immunosuppressive virulence factors, impairing neutrophil activation, potentially leading to immune evasion through alteration in innate immune response.
Corporate
University of Calgary
Faculty
Graduate Studies
Doi
http://dx.doi.org/10.11575/PRISM/27474
Uri
http://hdl.handle.net/11023/3418
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