Examining interactions between Pseudomonas aeruginosa and Staphylococcus aureus from cystic fibrosis lung infections
Date
2013-03-28
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Abstract
Pseudomonas aeruginosa and Staphylococcus aureus are two common cystic fibrosis (CF) pathogens, thought to interact within the lung and influence disease progression. This study on interspecies interactions revealed that P. aeruginosa mediates antagonistic interactions with S. aureus. Further analysis was completed to identify the mechanism of negative interactions between an S. aureus CF isolate, C105, and twenty seven P. aeruginosa CF isolates – nine of which were identified as inhibitors. It was demonstrated that an inhibitory factor is secreted by P. aeruginosa; is less than 5 kDa, can induce tobramycin resistance in C105 and reduce hemolytic activity suggesting the formation of small colony variants (SCVs). GC-MS analysis revealed that the primary inhibitory factor was not 4-hydroxy-2n-heptlyquinolone-N-oxide (HQNO) rather inhibition of S. aureus by P. aeruginosa is complex and involves the secretion of multiple factors. This research suggests that P. aeruginosa produces multiple anti-staphylococcal agents that play a paradoxical role as they inhibit growth yet allow for aminoglycoside resistance in S. aureus.
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Microbiology
Citation
Libertucci, J. (2013). Examining interactions between Pseudomonas aeruginosa and Staphylococcus aureus from cystic fibrosis lung infections (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26019