Temporal profile of blood cytokine levels and placental pathology in extremely preterm infants
Date
2020-04-26
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Objective: To evaluate the relationship between placental pathology and the temporal profiles of “cytokine” (cytokines, chemokines and growth factors) levels in extremely preterm infants. Study design: A prospective cohort study of preterm infants born before 29 weeks gestational age (GA) and admitted to the neonatal intensive care unit (NICU) between June 2017 and July 2018 (n=55). Levels of 27 cytokines were measured in blood obtained from the umbilical cord at birth, 24-72 hours, and 21-28 days of life. Cytokine levels were determined using the Bio-Rad Multiplex Immunoassay System. Placental pathology per the 2016 Amsterdam consensus criteria and grouped as normal (N), inflammation (I), vasculopathy (V), or combined vasculopathy and inflammation (V+I). Results: Complete data was available from 42 patients. Cord blood median levels of cytokines differed between groups with the highest levels observed in group V+I as compared to groups N, I and V for the following: Eotaxin (p = 0.038), G-CSF (p = 0.023), IFN-γ (p = 0.002), IL-1ra (p < 0.001), IL-4 (p = 0.005), IL-8 (p = 0.010), MCP-1 (p = 0.011), and TNFα (p = 0.002). Post hoc analysis revealed sex differences in group I, with higher median levels of FGF-basic (p = 0.03), G-CSF (p = 0.048), IL1b (p = 0.038), IL-1ra (p = 0.005), IL-8 (p = 0.005), MIP-1α (p = 0.048) and TNFα (p = 0.048) in females. Conclusion: Specific types of placental pathology may be associated with differential cytokine profiles in extremely preterm infants. Sampling from cord blood may help assess the pathological status of the placenta and potentially infer outcome risks for the infant.
Description
Keywords
Citation
Zein, H. (2020). Temporal profile of blood cytokine levels and placental pathology in extremely preterm infants (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.