The Evaluation of Conserved Amino Acids that Influence Polysubstrate Specificity in the Multidrug Resistance Transporter, EmrE

Simple item page
atmire.migration.oldid2385
dc.contributor.advisorTurner, Raymond
dc.contributor.authorSaleh, Marwah
dc.date.accessioned2014-08-21T20:18:31Z
dc.date.available2015-08-22T07:00:42Z
dc.date.issued2014-08-21
dc.date.submitted2014en
dc.description.abstractThe study of the molecular mechanism of substrate recognition/binding by multidrug resistance transporters has taken different approaches. I have utilized a PCR-driven site-directed mutagenesis analysis to alter conserved amino acid residues within the Escherichia coli small multidrug transporter, EmrE. The EmrE variants generated were further assessed for their resistance ability to 19 structurally different quaternary cationic compounds (QCC) using a high-throughput microtitre plate assay. The underlying hypothesis is that the mutation of conserved amino acids will alter the resistance profile of EmrE to structurally different QCC and identify the specificity of these residues to specific characteristic(s) of QCC. Based on 1,254 resistance profiles, the plasticity of EmrE binding pocket can be explained by the presence of conserved amino acid residues with different substrate preferences. The significance of studying EmrE is that it provides an excellent model for understanding the polysubstrate specificity on a molecular basis relatable to other major transporters.en_US
dc.description.embargoterms12 monthsen_US
dc.identifier.citationSaleh, M. (2014). The Evaluation of Conserved Amino Acids that Influence Polysubstrate Specificity in the Multidrug Resistance Transporter, EmrE (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28135en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28135
dc.identifier.urihttp://hdl.handle.net/11023/1691
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectGenetics
dc.subjectMicrobiology
dc.subjectBiochemistry
dc.subject.classificationMultidrug Resistanceen_US
dc.subject.classificationMultidrug transporteren_US
dc.subject.classificationSmall Multidrug Resistance Proteinsen_US
dc.subject.classificationEmrEen_US
dc.subject.classificationSite directed mutagenesisen_US
dc.titleThe Evaluation of Conserved Amino Acids that Influence Polysubstrate Specificity in the Multidrug Resistance Transporter, EmrE
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Ucalgary_2014_Saleh_Marwah.pdf
Size:
5.76 MB
Format:
Adobe Portable Document Format
Description:
Main article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.65 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Open Theses and Dissertations