Interactions of Inorganic Mercury and Inorganic Cadmium with Biomimetic and Complex Biological Membranes and their Influence on Membrane Packing and Size

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atmire.migration.oldid4533
dc.contributor.advisorPrenner, Elmar
dc.contributor.authorKerek, Evan
dc.contributor.committeememberGailer, Jürgen
dc.contributor.committeememberZaremberg, Vanina
dc.contributor.committeememberSutherland, Todd
dc.date.accessioned2016-06-21T18:11:15Z
dc.date.available2016-06-21T18:11:15Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractInorganic mercury (Hg2+) and Inorganic Cadmium (Cd2+) are toxic heavy metals linked to the development of cancer, diabetes and neurological dysfunctions. The effect of these metals on the fluidity and phase transition (Tm) of biomimetic and polar extract membranes was investigated using Laurdan Generalized Polarization (GP) and Dynamic Light Scattering (DLS). Hg2+ and Cd2+ electrostatically target and induce rigidity in membranes containing cationic and anionic lipids respectively. Hg2+ also imparts rigidity by acting as a catalyst in the vinyl ether hydrolysis of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) plasmalogens. Cd2+-induced rigidity of anionic membranes results in a stabilization of the gel phase and a suppression of the Tm of membranes composed of phosphatidic acid (PA), cardiolipin (CL), phosphatidylserine (PS), phosphatidylglycerol (PG) and phosphatidylinositol (PI). Cd2+ induces more rigidity in rigid anionic membranes compared to more fluid anionic membranes. These results further our understanding of metal-lipid interactions.en_US
dc.identifier.citationKerek, E. (2016). Interactions of Inorganic Mercury and Inorganic Cadmium with Biomimetic and Complex Biological Membranes and their Influence on Membrane Packing and Size (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26601en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26601
dc.identifier.urihttp://hdl.handle.net/11023/3066
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiochemistry
dc.subject.classificationMercuryen_US
dc.subject.classificationCadmiumen_US
dc.subject.classificationLipidsen_US
dc.subject.classificationLaurdanen_US
dc.subject.classificationFluorescenceen_US
dc.titleInteractions of Inorganic Mercury and Inorganic Cadmium with Biomimetic and Complex Biological Membranes and their Influence on Membrane Packing and Size
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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