The ecology and fitness consequences of gut microbiome variation in Sable Island feral horses (Equus caballus)

Stothart, Mason R.

The ecology and fitness consequences of gut microbiome variation in Sable Island feral horses (Equus caballus)

dc.contributor.advisor	Poissant, Jocelyn
dc.contributor.author	Stothart, Mason R.
dc.contributor.committeemember	Fox, Jeremy
dc.contributor.committeemember	McLoughlin, Philip Dunstan
dc.contributor.committeemember	Reimer De Bruyn, Raylene A
dc.contributor.committeemember	Wasmuth, James
dc.contributor.committeemember	Sycuro, Laura K
dc.contributor.committeemember	Archie, Elizabeth A
dc.date.accessioned	2023-09-18T15:17:10Z
dc.date.available	2023-09-18T15:17:10Z
dc.date.issued	2023-09-12
dc.description.abstract	Gut microbiomes are understood to be integral to the ecology and evolution of animal life, but until very recently, the scientific literature has lacked robust empirical characterization of the host genetic basis and fitness consequences of microbiome variation within wild animal populations. As a closed population of free-living but exhaustively surveyed fate-known individuals which are obligately reliant on their gut microbiomes, the feral horses of Sable Island (Nova Scotia) provide a tractable study system in which to study the ecology, host-to-host transmission, and fitness consequences of microbiome variation in the wild. In this thesis, I begin by characterizing the ecological determinants of the Sable Island horse hindgut microbiome (Chapter 2) and validate the use of shallow shotgun metagenomic sequencing methods for characterizing diverse microbial communities in the horse hindgut (Chapter 3). In applying a shallow shotgun metagenomic sequencing method to a dataset of 2394 fecal samples from 794 individuals spanning 7 years of collection, I find evidence that variation the gut microbiome is visible to host-level selection (Chapter 4). Further quantitative genetic analyses of these data reveal that microbiome features are weakly heritable on average (Chapter 5), suggesting a limited capacity for the microbiome to rapidly respond to selection; but animal model and eco-phylogenetic null model results independently provide evidence that the social dispersal of microbes between horses is an important determinant of microbiome structure. Furthermore, the microbiota which show the strongest evidence for social structuring are those which are most consequential for horse survival. These findings support hypotheses that non-genetic inheritance mechanisms (microbe dispersal) could critically underlie the ability for microbiomes to adaptively respond to selection on ecologically-relevant timescales. However, host inbreeding may constrain the capacity for microbiome variation to adaptively respond to host-level selection, since microbe responses to inbreeding were negatively associated with the estimated effects of those same microbiota on horse survival (Chapter 6). The proximate environmental and host genetic mechanisms underpinning microbiome variation, patterns of context-specific selection, and strain-level transmission remain to be characterized in detail. Nonetheless, this thesis contains tentative but important empirical milestones in describing the eco-evolutionary significance of host-associated microbiomes in the wild.
dc.identifier.citation	Stothart, M. R. (2023). The ecology and fitness consequences of gut microbiome variation in Sable Island feral horses (Equus caballus) (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.
dc.identifier.uri	https://hdl.handle.net/1880/117033
dc.language.iso	en
dc.publisher.faculty	Graduate Studies
dc.publisher.institution	University of Calgary
dc.rights	University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subject	holobiont
dc.subject	metagenomic
dc.subject	non-genetic inheritance
dc.subject	quantitative genetics
dc.subject	symbiosis
dc.subject	wildlife
dc.subject.classification	Education--Sciences
dc.subject.classification	Forestry and Wildlife
dc.subject.classification	Microbiology
dc.subject.classification	Ecology
dc.subject.classification	Biology--Molecular
dc.title	The ecology and fitness consequences of gut microbiome variation in Sable Island feral horses (Equus caballus)
dc.type	doctoral thesis
thesis.degree.discipline	Veterinary Medical Sciences
thesis.degree.grantor	University of Calgary
thesis.degree.name	Doctor of Philosophy (PhD)
ucalgary.thesis.accesssetbystudent	I require a thesis withhold – I need to delay the release of my thesis due to a patent application, and other reasons outlined in the link above. I have/will need to submit a thesis withhold application.