Development of the liver in the chicken embryo.  II. Erythropoietic and granulopoietic cells

Cavey, Michael J.; G. K., Wong,

Development of the liver in the chicken embryo. II. Erythropoietic and granulopoietic cells

dc.contributor.author	Cavey, Michael J.	en
dc.contributor.author	G. K., Wong,	en
dc.contributor.department	Biological Sciences	en
dc.contributor.faculty	Faculty of Science	en
dc.contributor.institution	University of Calgary	en
dc.date.accessioned	2006-08-14T19:49:13Z
dc.date.available	2006-08-14T19:49:13Z
dc.date.issued	1993
dc.description	Copyright held by publisher	en
dc.description.abstract	Hepatic hemopoiesis is apparent in the chicken embryo on day 7 of incubation (Hamburger and Hamilton Stage 301, and a peak in hemopoietic activity occurs on day 14 (Stage 40). During this period, the differentiation of hemopoietic cells was examined by light microscopy and by transmission and scanning electron microscopy. Glycol methacrylate sections were used in lieu of smears to study hemopoietic cells, thus minimizing the problems of cell shrinkage and rupture. The sections were superior to smears for close examination of nuclear and cytoplasmic morphologies and for precise localization of hemopoietic cells to intravascular and extravascular sites. The avian liver is involved directly with erythropoiesis and granulopoiesis only. Erythropoietic cells, occurring in intravascular and extravascular locations, appear throughout the time frame examined. Blood islands with granulopoietic cells were not observed until days 8-9 (Stage 35). Granulopoiesis in the liver produces only eosinophilic leukocytes. Individual granulopoietic cells appear first in the connective tissue sheaths of hepatic vessels, and these cells subsequently congregate into blood islands. Endothelial cells of the sinusoidal linings, through asymmetric divisions, frequently release daughter cells into the circulation, and Kupffer cells are actively engaged in phagocytosis of erythrocytes. From a comparative standpoint, the elements deemed critical to hemopoiesis in the mammalian liver-prehepatocyte population, hepatic vasculature, and compartments for stem cell differentiation-may not hold the same importance in the bird, owing to an inordinate reliance on intravascular hemopoiesis in this vertebrate class. Key words: Capillaries, Chick embryo, Vascular endothelium, Hematopoiesis, Kupffer cells, Liver, Microscopy, Morphogenesis o 1993 Wiley-Liss, Inc.	en
dc.description.refereed	yes	en
dc.format.extent	2088853 bytes
dc.format.mimetype	application/pdf
dc.identifier.citation	Wong, G. K., Cavey, M. J. "Development of the liver in the chicken embryo. II. Erythropoietic and granulopoietic cells" Anatomical Record 1993 235: 131–143	en
dc.identifier.doi	http://dx.doi.org/10.11575/PRISM/35100
dc.identifier.issn	1552-4884
dc.identifier.uri	http://hdl.handle.net/1880/43415
dc.language.iso	en	en
dc.publisher	John Wiley & Sons, Inc.	en
dc.publisher.url	http://www3.interscience.wiley.com/cgi-bin/jhome/28243
dc.subject	Biology	en
dc.subject.other	Capillaries	en
dc.subject.other	Chick embryo	en
dc.subject.other	Vascular endothelium	en
dc.subject.other	Hematopoiesis	en
dc.subject.other	Kupffer cells	en
dc.subject.other	Liver	en
dc.subject.other	Microscopy	en
dc.subject.other	Morphogenesis	en
dc.title	Development of the liver in the chicken embryo. II. Erythropoietic and granulopoietic cells	en
dc.type	journal article