Exercise and dietary interventions in a rat model of metabolic knee osteoarthritis

Rios, Jaqueline Lourdes

Exercise and dietary interventions in a rat model of metabolic knee osteoarthritis

dc.contributor.advisor	Herzog, Walter
dc.contributor.author	Rios, Jaqueline Lourdes
dc.contributor.committeemember	Hart, David D.
dc.contributor.committeemember	Reimer, Raylene A.
dc.contributor.committeemember	Krawetz, Roman J.
dc.contributor.committeemember	Beier, Frank
dc.date	2019-11
dc.date.accessioned	2019-09-09T18:34:32Z
dc.date.available	2019-09-09T18:34:32Z
dc.date.issued	2019-09-05
dc.description.abstract	Osteoarthritis is a debilitating chronic disease which has no cure or effective treatment. If no changes are made in prevention and treatment, osteoarthritis will continue to represent a significant economic burden to patients and society. The goal of this thesis was to determine the effects of moderate aerobic exercise and prebiotic fibre supplementation on the onset and progression of the metabolic knee osteoarthritis phenotype in rats exposed to a high-fat/high-sucrose diet. This study was divided into three phases. Phase 1: we evaluated the effects of a 12-week aerobic exercise program of varying duration on healthy rat knee cartilage. We determined that moderate, high and extra-high duration treadmill exercise has no detrimental effects on knee joint health, function and integrity. Therefore, we concluded that treadmill exercise at any tested duration was a safe exercise for rats in terms of knee osteoarthritis-like damage, and therefore, could be used as a safe exercise intervention in a pre-clinical rat model of knee osteoarthritis. Phase 2: we evaluated the effects of moderate aerobic exercise, prebiotic fibre supplementation, and the combination of exercise and fibre over a 12-week intervention in rats exposed to a high-fat/high-sucrose diet that has been shown to cause knee joint damage. Our findings indicated that prebiotic fibre and aerobic exercise prevented knee joint damage in this model. Phase 3: we evaluated if the damaging effects of the high-fat/high-sucrose diet could be delayed/reversed. We determined that neither prebiotic fibre supplementation nor aerobic exercise were able to stop the progression of existing knee osteoarthritis-like damage induced by a high-fat/high-sucrose diet. In summary, this thesis provides insight into two different approaches to prevent the development of metabolic osteoarthritis phenotype. However, the studies presented in this thesis were not able to show an effective way to stop the progression of the disease.	en_US
dc.identifier.citation	Rios, J. L. (2019). Exercise and dietary interventions in a rat model of metabolic knee osteoarthritis (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.	en_US
dc.identifier.doi	http://dx.doi.org/10.11575/PRISM/36965
dc.identifier.uri	http://hdl.handle.net/1880/110893
dc.language.iso	eng	en_US
dc.publisher.faculty	Kinesiology	en_US
dc.publisher.institution	University of Calgary	en
dc.rights	University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.	en_US
dc.subject	Osteoarthritis	en_US
dc.subject	Prebiotic Fibre	en_US
dc.subject	Exercise	en_US
dc.subject	Metabolic Syndrome	en_US
dc.subject	Rat Model	en_US
dc.subject.classification	Health Sciences	en_US
dc.title	Exercise and dietary interventions in a rat model of metabolic knee osteoarthritis	en_US
dc.type	doctoral thesis	en_US
thesis.degree.discipline	Kinesiology	en_US
thesis.degree.grantor	University of Calgary	en_US
thesis.degree.name	Doctor of Philosophy (PhD)	en_US
ucalgary.item.requestcopy	true	en_US