The Role of Calcium in the Mechanism of Muscle Fatigue in Intact, Single Muscle Fibres of Mice at Physiological Temperature

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atmire.migration.oldid5991
dc.contributor.advisorMacIntosh, Brian
dc.contributor.authorGlass, Lisa
dc.contributor.committeememberSyme, Doug
dc.contributor.committeememberMillet, Guillaume
dc.date.accessioned2017-09-20T15:38:33Z
dc.date.available2017-09-20T15:38:33Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractThe purpose of this study was to investigate the role of Ca2+ in the mechanism of fatigue during repeated submaximal contractions. Intact, mouse single muscle fibres were fatigued at 32C with intermittent submaximal stimulation until 40% of initial force. A force-[Ca2+]m relationship was determined prior to fatigue and during a 35 min recovery period. During fatigue, [Ca2+]m at 50% of maximum force (Ca50) increased significantly, demonstrating a decrease in Ca2+ sensitivity. Early in the period of intermittent contractions, Ca50 increased with an increase in [Ca2+]m followed by potentiation which was evident with a decrease in Ca50. However, Ca2+ sensitivity was never better than in the control condition. Throughout fatigue, both [Ca2+]m and sensitivity played a role in decreasing force. By 5 min of recovery, Ca50 had recovered and was not significantly different from Ca50 before fatigue. These results show that both [Ca2+]m and sensitivity play a role in fatigue.en_US
dc.identifier.citationGlass, L. (2017). The Role of Calcium in the Mechanism of Muscle Fatigue in Intact, Single Muscle Fibres of Mice at Physiological Temperature (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28320en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28320
dc.identifier.urihttp://hdl.handle.net/11023/4115
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyKinesiology
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectAnimal Physiology
dc.subject.otherforce
dc.subject.other[Ca2+]m
dc.subject.otherCa2+ sensitivity
dc.subject.othersubmaximal repeated contractions
dc.subject.othermuscle fatigue
dc.titleThe Role of Calcium in the Mechanism of Muscle Fatigue in Intact, Single Muscle Fibres of Mice at Physiological Temperature
dc.typemaster thesis
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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