Neutrophil Responses to Mixed-Species Anaerobic Biofilms

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atmire.migration.oldid2923
dc.contributor.advisorMorck, Douglas
dc.contributor.authorLockhart, Joey Scott
dc.date.accessioned2015-01-28T23:02:43Z
dc.date.available2015-02-23T08:00:40Z
dc.date.issued2015-01-28
dc.date.submitted2015en
dc.description.abstractBiofilms composed of anaerobic bacteria often result in persistent infections and chronic inflammation. Host immune cells have difficulties in clearing biofilm-related infections and this can result in unnecessary tissue damage due to the overproduction of proinflammatory mediators. The objective of this project was to generate mixed-species anaerobic biofilms composed of two opportunistic pathogens, Fusobacterium necrophorum and Porphyromonas levii and measure neutrophil responses. Neutrophils are a vital component of the innate immune system and the results show that neutrophils exposed to mixed-species planktonic bacteria exhibit a more extensive oxidative response than neutrophils exposed to biofilms composed of the same bacteria. The limited neutrophil response to biofilm bacteria may explain the reduced ability of the innate immune system to eradicate biofilm-associated infections. The results demonstrate that bacterial lipopolysaccharide plays a significant role in the stimulation of neutrophils, however the evidence points to the presence of other stimulatory molecules in the bacterial media.en_US
dc.identifier.citationLockhart, J. S. (2015). Neutrophil Responses to Mixed-Species Anaerobic Biofilms (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27088en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/27088
dc.identifier.urihttp://hdl.handle.net/11023/2039
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subjectImmunology
dc.subject.classificationNeutrophilen_US
dc.subject.classificationBiofilmen_US
dc.subject.classificationAnaerobeen_US
dc.subject.classificationOxidative Bursten_US
dc.titleNeutrophil Responses to Mixed-Species Anaerobic Biofilms
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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