Distribution and expression of protease genes in the Burkholderia cepacia complex

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dc.contributor.advisorSokol, Pamela A.
dc.contributor.authorGingues, Sonia
dc.date.accessioned2005-08-16T16:59:49Z
dc.date.available2005-08-16T16:59:49Z
dc.date.issued2004
dc.descriptionBibliography: p. 151-173en
dc.description.abstractThe distribution of the zinc metalloprotease gene, zmpA, and a sedolisin gene, scpB, was determined in the Burkholderia cepacia complex. Extracellular proteolytic activity correlated with the presence of zmpA with the exception of five strains that possess zmpA but are protease negative. To investigate the regulation of zmpA in these strains, zmpA::lacZ, zmpA::luxCDABE, cepl::luxCDABE and cepR::luxCDABE transcriptional fusions were constructed. zmpA, cepl and cepR genes are expressed in all the strains tested; however, different patterns of expression are observed. Protease activity of two of the five strains was restored by introduction of zmpA encoding an active protease. The amino acid sequence of the zinc metalloprotease from the two complemented strains was compared with that of an active protease. The two protease negative strains contain a serine to threonine substitution at position 489. ScpB mutants were constructed and scpB localized to the periplasm.en
dc.format.extentxiv, 173 leaves : ill. ; 30 cm.en
dc.identifier.citationGingues, S. (2004). Distribution and expression of protease genes in the Burkholderia cepacia complex (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/15768en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/15768
dc.identifier.isbn0612976475en
dc.identifier.lccAC1 .T484 2004 G56en
dc.identifier.urihttp://hdl.handle.net/1880/41542
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleDistribution and expression of protease genes in the Burkholderia cepacia complex
dc.typemaster thesis
thesis.degree.disciplineMicrobiology & Infectious Diseases
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 1503 520492020
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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