Leukocyte recruitment in contact sensitivity

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dc.contributor.advisorKubes, Paul
dc.contributor.authorHwang, John Muen
dc.date.accessioned2005-08-16T17:03:43Z
dc.date.available2005-08-16T17:03:43Z
dc.date.issued2004
dc.descriptionBibliography: p. 105-120en
dc.descriptionSome pages are in colour.en
dc.description.abstractContact sensitivity is an inflammatory disorder characterized by leukocyte recruitment. We used intravital microscopy to directly visualize leukocyte rolling and adhesion. By blocking specific adhesion molecules, we elucidated the molecular mechanisms mediating early leukocyte recruitment to be E- and P-selectin and then demonstrated that leukocyte recruitment in the late phase had a different adhesive profile (mainly a4- integrin). Complete blockade of E- and P-selectin within the first 2 hours of leukocyte­endothelial cell interactions eliminated selectin-independent leukocyte recruitment at 24 hours. Specific elimination of CD4, lymphocytes in the early phase eliminated the late response. Addition of these same CD4+ lymphocytes two hours after antigen challenge was too late for these cells to home to the skin. We further established that mast cells act to modulate the T-lymphocyte response in CS. In the absence of mast cells, T1-1l cells preferentially adhere to vascular endothelium, whereas T112 cells preferentially adhere in wild-type mice.en
dc.format.extentx, 120 leaves : ill. ; 30 cm.en
dc.identifier.citationHwang, J. M. (2004). Leukocyte recruitment in contact sensitivity (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/16049en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/16049
dc.identifier.urihttp://hdl.handle.net/1880/41619
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleLeukocyte recruitment in contact sensitivity
dc.typemaster thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 1508 520492025
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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