Calcium Regulation of a Slow AHP in Hippocampal Pyramidal Cells

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dc.contributor.advisorTurner, Raymond Joseph
dc.contributor.authorMiclat, Jason
dc.contributor.committeememberWhelan, Patrick J.
dc.contributor.committeememberZamponi, Gerald W.
dc.date2018-06
dc.date.accessioned2018-04-13T14:58:49Z
dc.date.available2018-04-13T14:58:49Z
dc.date.issued2018-04-12
dc.description.abstractA slow afterhyperpolarization (sAHP) in CA1 hippocampal pyramidal cells is calcium-dependent and involves activation of KCa3.1 and KCNQ potassium channels. Both KCa3.1 and KCNQ channels bind calmodulin (CaM) as a calcium sensor, with an additional role proposed for the protein hippocalcin. We measured the calcium sensitivity of the IsAHP and potassium channel associations with CaM or hipppocalcin. In recordings in hippocampal tissue slices in vitro the KCa3.1-mediated IsAHP steadily increased over 10 min equilibration to 0 µM calcium electrolyte, but decreased upon infusing 1 µM calcium, while the KCNQ-mediated IsAHP responded in an opposite manner. KCa3.1 channels contributed to the IsAHP in rat but not mouse pyramidal cells. Coimmunoprecipitation occurred between KCa3.1 and both CaM and hippocalcin, but only KCNQ and CaM. These data reveal a species-specific expression pattern for two potassium channels and in calcium sensor proteins present to generate the sAHP in the same class of hippocampal neuron.en_US
dc.identifier.citationMiclat, J. (2018). Calcium Regulation of a Slow AHP in Hippocampal Pyramidal Cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/31781en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/31781
dc.identifier.urihttp://hdl.handle.net/1880/106493
dc.language.isoeng
dc.publisher.facultyCumming School of Medicine
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectslow afterhyperpolarization
dc.subjecthippocampus
dc.subjectCA1
dc.subjecthippocalcin
dc.subjectcalmodulin
dc.subjectKCa3.1
dc.subjectKCNQ
dc.subject.classificationNeuroscienceen_US
dc.titleCalcium Regulation of a Slow AHP in Hippocampal Pyramidal Cells
dc.typemaster thesis
thesis.degree.disciplineNeuroscience
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.checklistI confirm that I have submitted all of the required forms to Faculty of Graduate Studies.en_US
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