Nkx3.2 regulates zebrafish vascular stabilization through recruitment of mural cells

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atmire.migration.oldid2834
dc.contributor.advisorChilds, Sarah
dc.contributor.authorSpice, Peter James
dc.date.accessioned2015-01-08T16:28:51Z
dc.date.available2015-02-23T08:00:37Z
dc.date.issued2015-01-08
dc.date.submitted2014en
dc.description.abstractVascular stabilization is the process during vascular development where the immature endothelial cell tube gains structural support through the recruitment and adhesion of perivascular mural cells; pericytes and vascular smooth muscle cells (vSMC). When vessels are not well supported by mural cells hemorrhage can occur. Nkx3.2 deficient zebrafish embryos hemorrhage, have reduced expression of a mural cell marker, and the endothelial layer makes less contact with mural cells compared with controls. I found that nkx3.2 regulates the expression of two genes with known roles in neural crest migration and specification; pdgfrα and sox10. Further, nkx3.2 functions autonomously in neural crest cells, where knockdown of nkx3.2 causes abnormal migration of neural crest cells in the head. These results show that nkx3.2 regulates genes involved in neural crest migration and in turn supports recruitment of mural cells to mediate vascular stabilization.en_US
dc.identifier.citationSpice, P. J. (2015). Nkx3.2 regulates zebrafish vascular stabilization through recruitment of mural cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27107en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/27107
dc.identifier.urihttp://hdl.handle.net/11023/1994
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyMedicine
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology--Cell
dc.subject.classificationzebrafishen_US
dc.subject.classificationmural cellen_US
dc.subject.classificationendothelial cellen_US
dc.subject.classificationVascular developmenten_US
dc.subject.classificationstabilizationen_US
dc.titleNkx3.2 regulates zebrafish vascular stabilization through recruitment of mural cells
dc.typemaster thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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