The Role of L- and T-type Ca2+ Channels in Rat Cerebral Arteries

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dc.contributor.advisorKargacin, Gary
dc.contributor.authorAbd El-Rahman, Rasha
dc.date.accessioned2013-12-04T22:48:12Z
dc.date.available2014-03-15T07:00:15Z
dc.date.issued2013-12-04
dc.date.submitted2013en
dc.description.abstractThe overall goal of this thesis was to identify which voltage-gated Ca2+ channels are expressed in rat cerebral arterial smooth muscle and to determine their contributions to myogenic tone regulation. We began by exploring which voltage-gated Ca2+ channels are expressed in cerebral arterial smooth muscle. A combination of molecular, electrophysiological and functional measurements revealed the presence of L- (CaV1.2) and T-type (CaV3.1 and CaV3.2) Ca2+ channel subtypes in rat cerebral arteries. Both types contribute to arterial tone development, although the contribution of the L-type channels to tone development is greater. We then investigated the role of a specific T-type Ca2+ channel subtype, CaV3.2, in cerebral arterial smooth muscle by functional assessment and a structural approach using immunohistochemistry, proximity ligation assay, electron-tomography, and immunogold labeling, combined with computational modeling and electrophysiological measurements. Results indicate that Ca2+ influx through CaV3.2 channels elicits dilation by activating ryanodine receptors and inducing Ca2+ sparks, localized events that activate BKCa channels. In conclusion this work provided evidence for the presence of different types of voltage-gated Ca2+ channels and provided evidence of their diverse functional roles in regulating myogenic tone in rat cerebral arteries. Overall, the conclusions indicate the importance of the different functional roles of voltage-gated Ca2+ channels, which have substantial physiological relevance to the function of the cerebral vasculature.en_US
dc.identifier.citationAbd El-Rahman, R. (2013). The Role of L- and T-type Ca2+ Channels in Rat Cerebral Arteries (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28330en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28330
dc.identifier.urihttp://hdl.handle.net/11023/1172
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology--Cell
dc.subjectBiology--Molecular
dc.subjectPhysiology
dc.subject.classificationVoltage-gated Ca2+ channelsen_US
dc.subject.classificationCa2+ regulationen_US
dc.subject.classificationVascular smooth muscle cellsen_US
dc.titleThe Role of L- and T-type Ca2+ Channels in Rat Cerebral Arteries
dc.typedoctoral thesis
thesis.degree.disciplineCardiovascular & Respiratory Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameDoctor of Philosophy (PhD)
ucalgary.item.requestcopytrue
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