The Role of L- and T-type Ca2+ Channels in Rat Cerebral Arteries
atmire.migration.oldid | 1645 | |
dc.contributor.advisor | Kargacin, Gary | |
dc.contributor.author | Abd El-Rahman, Rasha | |
dc.date.accessioned | 2013-12-04T22:48:12Z | |
dc.date.available | 2014-03-15T07:00:15Z | |
dc.date.issued | 2013-12-04 | |
dc.date.submitted | 2013 | en |
dc.description.abstract | The overall goal of this thesis was to identify which voltage-gated Ca2+ channels are expressed in rat cerebral arterial smooth muscle and to determine their contributions to myogenic tone regulation. We began by exploring which voltage-gated Ca2+ channels are expressed in cerebral arterial smooth muscle. A combination of molecular, electrophysiological and functional measurements revealed the presence of L- (CaV1.2) and T-type (CaV3.1 and CaV3.2) Ca2+ channel subtypes in rat cerebral arteries. Both types contribute to arterial tone development, although the contribution of the L-type channels to tone development is greater. We then investigated the role of a specific T-type Ca2+ channel subtype, CaV3.2, in cerebral arterial smooth muscle by functional assessment and a structural approach using immunohistochemistry, proximity ligation assay, electron-tomography, and immunogold labeling, combined with computational modeling and electrophysiological measurements. Results indicate that Ca2+ influx through CaV3.2 channels elicits dilation by activating ryanodine receptors and inducing Ca2+ sparks, localized events that activate BKCa channels. In conclusion this work provided evidence for the presence of different types of voltage-gated Ca2+ channels and provided evidence of their diverse functional roles in regulating myogenic tone in rat cerebral arteries. Overall, the conclusions indicate the importance of the different functional roles of voltage-gated Ca2+ channels, which have substantial physiological relevance to the function of the cerebral vasculature. | en_US |
dc.identifier.citation | Abd El-Rahman, R. (2013). The Role of L- and T-type Ca2+ Channels in Rat Cerebral Arteries (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28330 | en_US |
dc.identifier.doi | http://dx.doi.org/10.11575/PRISM/28330 | |
dc.identifier.uri | http://hdl.handle.net/11023/1172 | |
dc.language.iso | eng | |
dc.publisher.faculty | Graduate Studies | |
dc.publisher.institution | University of Calgary | en |
dc.publisher.place | Calgary | en |
dc.rights | University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. | |
dc.subject | Biology--Cell | |
dc.subject | Biology--Molecular | |
dc.subject | Physiology | |
dc.subject.classification | Voltage-gated Ca2+ channels | en_US |
dc.subject.classification | Ca2+ regulation | en_US |
dc.subject.classification | Vascular smooth muscle cells | en_US |
dc.title | The Role of L- and T-type Ca2+ Channels in Rat Cerebral Arteries | |
dc.type | doctoral thesis | |
thesis.degree.discipline | Cardiovascular & Respiratory Sciences | |
thesis.degree.grantor | University of Calgary | |
thesis.degree.name | Doctor of Philosophy (PhD) | |
ucalgary.item.requestcopy | true |