Investigation into Equine Papillomavirus Type 2 Prevalence and Diagnosis
Date
2018-09-28
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Abstract
Equine papillomavirus type 2 (EcPV-2) has been consistently associated with most equine genital cancers and a subset of cancers at other locations. The prevalence of EcPV-2 in healthy horses has been estimated to be <20% for DNA presence, and 10-36% for anti-EcPV-2-L1 seroprevalence. However, no study has thus far examined the prevalence of EcPV-2 at the herd-level or over multiple timepoints. This thesis examined EcPV-2 prevalence in a predominantly closed herd with a known EcPV-2 infected horse over three timepoints. We found that DNA prevalence (0-4%) was equivalent to the general horse population, but that seroprevalence (51.5%) was higher. Additionally, no study has examined the distribution of EcPV-2 infection in situ, nor compared such a technique to PCR. We found that RNA ISH concorded with PCR in 80% of cases. We also recapitulated previous results concerning EcPV-2 distribution in situ as well as found EcPV-2 in numerous novel locations.
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Keywords
EcPV-2, equine papillomavirus type 2
Citation
Wachoski-Dark, G. L. (2018). Investigation into Equine Papillomavirus Type 2 Prevalence and Diagnosis (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/33154