The Evaluation of MS4A4A and MS4A8B Expression in Hematopoietic Cells

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atmire.migration.oldid2474
dc.contributor.advisorDeans, Julie
dc.contributor.authorPuri, Mandip
dc.date.accessioned2014-09-23T21:21:06Z
dc.date.available2014-11-17T08:00:47Z
dc.date.issued2014-09-23
dc.date.submitted2014en
dc.description.abstractMS4A is a family of proteins with four membrane spanning regions. Currently, among the 15+ human MS4A family members, most remain uncharacterized. A notable exception is MS4A1 (CD20) which displays restricted expression on the surface of B-lymphocytes and forms the immunotherapeutic target of Rituximab. Given the importance of MS4A1 as an immunotherapeutic target our goal was to search for additional members of the MS4A family that may form potential immunotherapeutic targets. Here, I describe the evaluation of I) MS4A8B and MS4A4A expression in select hematopoietic cell subsets, using monoclonal antibodies that were generated against extracellular epitopes, and II) the investigation of whether monoclonal antibodies directed against MS4A4A can induce Fc-receptor independent effects characteristic of immunotherapeutic antibodies such as cellular apoptosis. We found that MS4A8B is not expressed in any of the hematopoietic cell subsets evaluated but is present in the NCI-H69 small cell lung carcinoma cell line. However, we identified that MS4A4A is expressed in select hematopoietic cell subsets. Specifically, herein I report that MS4A4A is expressed in peripheral blood monocytes and monocyte derived M2 macrophages but not resting peripheral blood granulocytes, lymphocytes, or M1 macrophages. I also report that monoclonal antibodies directed against MS4A4A were not capable of inducing cellular apoptosis or influencing select cellular functions in the cells evaluated.en_US
dc.identifier.citationPuri, M. (2014). The Evaluation of MS4A4A and MS4A8B Expression in Hematopoietic Cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28136en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28136
dc.identifier.urihttp://hdl.handle.net/11023/1791
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology
dc.subjectBiology--Molecular
dc.subjectImmunology
dc.subject.classificationMS4Aen_US
dc.subject.classificationMS4A4Aen_US
dc.subject.classificationMS4A8Ben_US
dc.subject.classificationM2 macrophageen_US
dc.subject.classificationM1 macrophageen_US
dc.subject.classificationAlternatively activated macrophageen_US
dc.subject.classificationClassically activated macrophageen_US
dc.titleThe Evaluation of MS4A4A and MS4A8B Expression in Hematopoietic Cells
dc.typemaster thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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