Seasonally Related Metabolic Profile and Regulation during Growth and Reproduction in Goldfish

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dc.contributor.advisorHabibi, Hamid
dc.contributor.authorLadisa, Claudia
dc.contributor.committeememberLewis, Ian
dc.contributor.committeememberRo, Dae-Kyun
dc.date2022-06
dc.date.accessioned2022-05-04T17:19:32Z
dc.date.available2022-05-04T17:19:32Z
dc.date.issued2022-04
dc.description.abstractMany fish species are seasonal breeders and undergo an annual cycle of predominant growth and reproductive phases. Seasonal variations are controlled by hormones of the brain-pituitary-peripheral axis and involve changes in gonadal development, growth, and metabolism. Previous studies have demonstrated that the hypothalamic neurohormones gonadotropin-releasing hormone (GnRH) and gonadotropin-inhibitory hormone (GnIH) are among the key regulatory factors involved in the multifactorial control of growth and reproduction in vertebrates. In oviparous species such as fish, significant metabolic energy is needed during reproduction in both female and male to produce hundreds of eggs and millions of sperm. The main goal of my dissertation was to investigate metabolic changes and patterns of energy allocation during growth and reproduction in the goldfish (Carassius auratus). Moreover, I tested the hypothesis that GnRH and GnIH are involved in the regulation of metabolism by investigating the seasonally-related effects of these peptides. An in vivo metabolomics approach was used to investigate the metabolic profile and regulation in male and female goldfish at different stages of the reproductive cycle. Two Liquid Chromatography-Mass Spectrometry (LC-MS) based approaches - metabolic profiling and isotope tracing - provided evidence for distinct patterns of energy allocation during reproduction and growth in goldfish. The findings demonstrate significant differences in the hepatic metabolic profile of three distinct reproductive stages in male and female fish involving carbohydrate, protein, lipid and nucleotide metabolism. Furthermore, the results presented in this study prove that GnRH and GnIH can modulate hepatic metabolism in a sexually-dimorphic and seasonally dependent manner. Tracer-based metabolomics using [U-13C]glucose demonstrate that the activity of pathways of the central carbon metabolism in the liver, gonads and muscle of male and female goldfish are different between growth and reproductive stages. Finally, the findings reveal sex specific differences and similarities in the regulation of metabolism during the annual cycle. Together, the results presented in this dissertation provide novel information and a framework to better understand the hormonally induced metabolic changes that accompany growth and reproduction in fish and other species undergoing seasonal cycles.en_US
dc.identifier.citationLadisa, C. (2022). Seasonally related metabolic profile and regulation during growth and reproduction in goldfish (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/39733
dc.identifier.urihttp://hdl.handle.net/1880/114613
dc.language.isoengen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectMetabolomicsen_US
dc.subjectGrowth and reproductionen_US
dc.subjectMetabolismen_US
dc.subjectEnergy allocationen_US
dc.subjectFish physiologyen_US
dc.subjectGoldfishen_US
dc.subject.classificationAnimal Physiologyen_US
dc.subject.classificationBioinformaticsen_US
dc.subject.classificationBiology--Molecularen_US
dc.titleSeasonally Related Metabolic Profile and Regulation during Growth and Reproduction in Goldfishen_US
dc.typedoctoral thesisen_US
thesis.degree.disciplineBiological Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameDoctor of Philosophy (PhD)en_US
ucalgary.item.requestcopytrueen_US
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