The Effects of Oxidative Stress on Neuronal Membrane Permeability and Redox Sensitive Transport Mechanisms

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atmire.migration.oldid5853
dc.contributor.advisorWildering, Willem
dc.contributor.authorPerry, Alexander
dc.contributor.committeememberSyme, Douglas
dc.contributor.committeememberPrenner, Elmar
dc.contributor.committeememberAntle, Michael
dc.date.accessioned2017-08-17T20:09:55Z
dc.date.available2017-08-17T20:09:55Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractLipid peroxidation is a symptom of aging that is suspected to cause undesirable alterations to membrane properties. These alterations are postulated to induce membranes defects, which negatively affect aspects of cells and hinders their functionality. Neuronal plasma membranes contain constituents that are particularly susceptible to oxidative species; therefore, they are prime targets for lipid peroxidation. Neurons from Lymnaea stagnalis were used in combination with various fluorescent probes to assess membrane hydrophobic barrier function to exclude the entry of ionic molecules through unassisted means. However, instead of exclusively probing passive permeability, it was discovered that various forms of active membrane transport processes in neuronal membranes may also be regulated by redox reactions. Contrary to suggestions from modeling studies, lipid peroxidation appeared to only slightly alter passive membrane permeability, suggesting that biological membranes may contain certain constituents and/or mechanisms that prevents lipid peroxidation from being as detrimental as observed under artificial conditions.en_US
dc.identifier.citationPerry, A. (2017). The Effects of Oxidative Stress on Neuronal Membrane Permeability and Redox Sensitive Transport Mechanisms (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28110en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28110
dc.identifier.urihttp://hdl.handle.net/11023/4023
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectEducation--Sciences
dc.subjectAnimal Physiology
dc.subjectBiology--Cell
dc.subjectNeuroscience
dc.subjectPhysiology
dc.subjectZoology
dc.subject.otherOxidative stress
dc.subject.otheraging
dc.subject.otherLymnaea stagnalis
dc.subject.otherNeuron
dc.subject.otherCell culture
dc.subject.otherABC transporter
dc.subject.otherMulti-drug resistance-associated protein
dc.subject.otherROS
dc.subject.otherPlasma membrane
dc.subject.otherEndocytosis
dc.subject.otherLysosomes
dc.subject.otherPropidium iodide
dc.subject.otherCarboxyfluorescein
dc.subject.otherProbenecid
dc.subject.otherFluorescein diacetate
dc.titleThe Effects of Oxidative Stress on Neuronal Membrane Permeability and Redox Sensitive Transport Mechanisms
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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