Enhancing the Production of Lipids and Antioxidants in Heterotrophic Cultures of Auxenochlorella protothecoides

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atmire.migration.oldid5115
dc.contributor.advisorDe la Hoz Siegler, Hector
dc.contributor.authorIbarra Vidal, Mary
dc.contributor.committeememberKallos, Michael
dc.contributor.committeememberChu, Angus
dc.date.accessioned2016-11-15T17:46:32Z
dc.date.available2016-11-15T17:46:32Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractAuxenochlorella protothecoides, aside its potential for biofuels, is an excellent source of antioxidants, reaching high lipid productivity under heterotrophic conditions utilizing glucose. Glucose-fed cultures, however, experience chloroplast bleaching affecting antioxidants production. In this study, glycerol, was evaluated as a carbon source at varying carbon-nitrogen ratios in batch-cultures. Glycerol presence in glucose fed-cultures reduced, or completely eliminated, chloroplast bleaching and increased the substrate-to-biomass yield beyond its thermodynamic limit. These results, suggest that glycerol forced the photosynthetic pathway to remain active, resulting in dark carbon fixation through the Calvin-cycle reactions. The maximum cell-density and biomass productivity were 17.60 g/L and 1.91 g/L·d respectively. Cultures grown on only-glycerol showed the highest lipid yield, 149 mg-lipids/g-substrate and antioxidants yield 6.650 µmol-Trolox/g-substrate. These values were higher to those observed with sole-glucose fed-cultures by 7% and 93% respectively. The maximum antioxidant productivity reached was 12.53 µM-TEAC/L•d, value comparable to those previously reported for Haematococcus Pluvialis.en_US
dc.identifier.citationIbarra Vidal, M. (2016). Enhancing the Production of Lipids and Antioxidants in Heterotrophic Cultures of Auxenochlorella protothecoides (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25910en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25910
dc.identifier.urihttp://hdl.handle.net/11023/3456
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectEngineering--Chemical
dc.subject.classificationmicroalgaeen_US
dc.subject.classificationprotothecoidesen_US
dc.subject.classificationLipidsen_US
dc.subject.classificationAntioxidantsen_US
dc.subject.classificationglycerolen_US
dc.titleEnhancing the Production of Lipids and Antioxidants in Heterotrophic Cultures of Auxenochlorella protothecoides
dc.typemaster thesis
thesis.degree.disciplineChemical and Petroleum Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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