Clostridium difficile infection susceptibility is controlled by alterations to the gut microbiota before and after antibiotic exposure

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atmire.migration.oldid2376
dc.contributor.advisorBeck, Paul
dc.contributor.advisorMacDonald, Justin
dc.contributor.authorSchenck, Louis Patrick
dc.date.accessioned2014-07-30T18:07:23Z
dc.date.embargolift2016-07-29T18:07:23Z
dc.date.issued2014-07-30
dc.date.submitted2014en
dc.description.abstractClostridium difficile (Cdif) infections (CDI) cause devastating diarrheal disease. Small clinical trials have generated excitement for the use of fecal microbial transplants as a therapeutic option; however, the exact components of the microbiota needed for protection against CDI have remained elusive. C57Bl/6 mice from two vendors, Jackson (JAX) and Charles River (CRV), were exposed to antibiotics before gavage with Cdif spores. JAX mice developed severe CDI as evidenced by weight loss, histological damage, and increased pro-inflammatory cytokines, whereas CRV mice were resistant to CDI. There were significant differences in the gut microbiota between the mice, but CRV mice retained a high abundance of Parabacteroides after antibiotics. Following cohousing, JAX mice had less severe CDI and CRV mice had more severe CDI. Transfer of P. distasonis, a CRV commensal bacteria, was able to dampen effects of CDI in JAX mice. This study may lead to more targeted bacteriotherapy for the treatment and prevention of CDI.en_US
dc.description.embargoterms2 yearsen_US
dc.identifier.citationSchenck, L. P. (2014). Clostridium difficile infection susceptibility is controlled by alterations to the gut microbiota before and after antibiotic exposure (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25337en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25337
dc.identifier.urihttp://hdl.handle.net/11023/1661
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subjectImmunology
dc.subject.classificationclostridiumen_US
dc.subject.classificationdifficileen_US
dc.subject.classificationparabacteroidesen_US
dc.subject.classificationdistasonisen_US
dc.subject.classificationmouse vendoren_US
dc.subject.classificationmicrobiotaen_US
dc.subject.classificationGut Microbiotaen_US
dc.titleClostridium difficile infection susceptibility is controlled by alterations to the gut microbiota before and after antibiotic exposure
dc.typemaster thesis
thesis.degree.disciplineGastrointestinal Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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