Minocycline treatment timing and its influence on serotonin expression following spinal cord injury

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dc.contributor.advisorWhelan, Patrick J.
dc.contributor.authorFlood, Jennifer Margaret
dc.contributor.committeememberTrang, Tuan
dc.contributor.committeememberCasha, Steven
dc.contributor.committeememberOusman, Shalina S.
dc.date2020-02
dc.date.accessioned2019-12-02T22:26:16Z
dc.date.available2019-12-02T22:26:16Z
dc.date.issued2019-11
dc.description.abstractThe effects of incomplete traumatic spinal cord injury (SCI) can be partly reversed by the plasticity of local and spared descending projections. A promising window of plasticity occurs for a number of weeks following injury and involves the control of neuroinflammatory processes. The FDA-approved drug, minocycline, is a promising drug for treating SCI since it decreases microglia activity, reduces macrophage activity, and generally provides neuroprotective properties. In this thesis I established a timeline of injury, looking at both serotonin (5-HT) and microglia/macrophage (Iba-1) immunoreactivity (ir), and I targeted a time point before a significant reduction of descending serotonergic fibers, in the form of 5-HTir, took place (i.e. 1-week). I found that the administration of minocycline increased 5-HTir caudal and ipsilateral to the lesion, compared to shams and controls. Using the selected time point, 1-week post-SCI, I administered minocycline and found a decrease in lesion size and an increase of 5-HTir both caudal and ipsilateral to the injury as well as rostral and contralateral to the injury. In this thesis, I provide evidence that minocycline impacts 5-HT expression when administered acutely and one week following SCI. These data suggest that the timing of minocycline treatment influences the neuroprotective properties previously reported and also influences descending 5-HT expression post-SCI.en_US
dc.identifier.citationFlood, J. M. (2019). Minocycline treatment timing and its influence on serotonin expression following spinal cord injury (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/37289
dc.identifier.urihttp://hdl.handle.net/1880/111278
dc.language.isoengen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjecttraumatic spinal cord injury, spinal cord injury, serotonin, immunoreactivity, neuroinflammation, minocycline, microglia, macrophage, neuroprotective, lesion, plasticity, hemisectionen_US
dc.subject.classificationEducation--Sciencesen_US
dc.subject.classificationNeuroscienceen_US
dc.titleMinocycline treatment timing and its influence on serotonin expression following spinal cord injuryen_US
dc.typemaster thesisen_US
thesis.degree.disciplineMedicine – Neuroscienceen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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