Functional characterization of murine cd20

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dc.contributor.advisorDeans, Julie
dc.contributor.authorMorsy, Deyaa El Deen
dc.date.accessioned2017-12-18T22:30:29Z
dc.date.available2017-12-18T22:30:29Z
dc.date.issued2012
dc.descriptionBibliography: p. 189-232en
dc.descriptionA few pages are in colour.en
dc.description.abstractCD20 is a tetraspanning membrane protein that is expressed on B lymphocytes, and functions in B cell receptor (BCR)-mediated calcium entry. The effect of murine CD20 deficiency on calcium mobilization was examined in two previous studies, with inconsistent findings. Humoral immunity induced in the context of murine CD20 deficiency was characterized by a reduction in T -independent responses, but normal TĀ­dependent (TD) immune responses. In the current study, I examined the in vitro calcium responses of murine CD20-1- B cells, and the in vivo TD immune responses of CD20-1- mice. I confirmed that BCR-mediated calcium mobilization was reduced in CD20-1- B cells following BCR stimulation. However, calcium responses following CD 19 ligation, and following BCR-CD 19 co-ligation were enhanced, suggesting an indirect role for CD20 in calcium entry rather than direct involvment as a calcium channel. TD immune responses against adeno-associated virus (AA V) and sheep red blood cells (SRBC) were characterized by reduced neutralizing antibody response to AA V, and reduced primary (IgM) and secondary (IgGl and IgG2b) responses to SRBC. The anti-SRBC response was associated with reduced germinal center B cell numbers. Further evaluation of B cells from unimmunized CD20-1- mice revealed increased CD20-1- B cell adhesion, enhanced LF A-1 expression and activation, and enhanced transmigration. The current data characterize a new phenotype for murine CD20 deficiency, and support a role for CD20 in in vivo B cell function.
dc.format.extentxviii, 232 leaves : ill. ; 30 cm.en
dc.identifier.citationMorsy, D. E. (2012). Functional characterization of murine cd20 (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4704en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/4704
dc.identifier.urihttp://hdl.handle.net/1880/105705
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleFunctional characterization of murine cd20
dc.typedoctoral thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameDoctor of Philosophy (PhD)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 2091 627942963
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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