Assessing Ryanodine Receptor Inhibition and Antioxidant Ability of Carvedilol and its Abilities

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atmire.migration.oldid4668
dc.contributor.advisorBack, Thomas George
dc.contributor.authorMalig, Thomas
dc.contributor.committeememberLing, Chang-Chun
dc.contributor.committeememberDerksen, Darren
dc.contributor.committeememberHeyne, Belinda
dc.date.accessioned2016-07-25T16:40:05Z
dc.date.available2016-07-25T16:40:05Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractThe antioxidant ability of the commonly prescribed antiarrhythmic medication carvedilol was assessed using two distinct assays. The first assay monitored the depletion of the stable radical DPPH via hydrogen atom abstraction utilizing UV-VIS spectrophotometry. The second assay involved monitoring the inhibition of a radical chain reaction initiated by UV light. Three metabolites of carvedilol were synthesized and assessed in each assay along with carvedilol and several benchmark antioxidants to ensure assay validity. It was determined that carvedilol possessed negligible antioxidant ability in both assays, while the metabolites possessed moderate-high antioxidant strength. It is therefore concluded that the antioxidant ability of carvedilol originates from the phenolic metabolites and not from carvedilol itself. The primary function of carvedilol to regulate calcium handling in cardiac myocytes was also assessed for each metabolite using a mutant embryonic cell line. It was determined that metabolic deactivation via hydroxylation pathways is minimal.en_US
dc.identifier.citationMalig, T. (2016). Assessing Ryanodine Receptor Inhibition and Antioxidant Ability of Carvedilol and its Abilities (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25020en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25020
dc.identifier.urihttp://hdl.handle.net/11023/3151
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectChemistry--Organic
dc.subject.classificationMetabolitesen_US
dc.subject.classificationAntioxidantsen_US
dc.subject.classificationCarvedilolen_US
dc.titleAssessing Ryanodine Receptor Inhibition and Antioxidant Ability of Carvedilol and its Abilities
dc.typemaster thesis
thesis.degree.disciplineChemistry
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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