Developing large-scale bioreactor protocols to expand pancreatic endocrine tissue to treat type 1 diabetes

Simple item page
dc.contributor.advisorBehie, Leo A.
dc.contributor.authorChawla, Meera
dc.date.accessioned2005-08-16T16:53:01Z
dc.date.available2005-08-16T16:53:01Z
dc.date.issued2004
dc.descriptionBibliography: p. 254-279en
dc.descriptionSome pages are in colour.en
dc.description.abstractType 1 diabetes is a disease of the pancreas in which the beta islet (endocrine) cells, which are responsible for insulin production, are destroyed by the immune system. The expansion in bioreactors and subsequent transplantation of endocrine cells could revolutionize the treatment for Type 1 diabetes using the "world-famous" Edmonton Protocol. Consequently, the aim of this study was to develop a scaleable process to expand mammalian pancreatic endocrine tissue in suspension bioreactors. Issues regarding cell culture medium conditions and cell handling protocols were investigated. Experiments in suspension bioreactors for 9 days showed a greater than 7-fold increase in the number of insulin-positive cells. Furthermore, aggregates were islet-like, as all of the endocrine cell types were present. Most importantly, cells exhibited glucose-responsive behavior and thus functionality.. These results represent a major milestone on the path to effective expansion and clinical use of bioreactor-produced islet-like structures in the treatment of Type 1 diabetes.en
dc.format.extentxxiii, 279 leaves : ill. ; 30 cm.en
dc.identifier.citationChawla, M. (2004). Developing large-scale bioreactor protocols to expand pancreatic endocrine tissue to treat type 1 diabetes (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/23736en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/23736
dc.identifier.urihttp://hdl.handle.net/1880/41400
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleDeveloping large-scale bioreactor protocols to expand pancreatic endocrine tissue to treat type 1 diabetes
dc.typemaster thesis
thesis.degree.disciplineChemical and Petroleum Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 1494 520492011
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2004_Chawla.pdf
Size:
39.4 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Legacy Theses